宏基因组学与液相色谱-串联质谱联用分析揭示两种典型中国传统鱼类制品（糟制鳜鱼和半干大黄鱼）中产生物胺的菌株
Integrated Metagenomic and LC-MS/MS Analysis Reveals the Biogenic Amine-Producing Strains of Two Typical Chinese Traditional Fish Products: Fermented Mandarin Fish () and Semi-Dried Yellow Croaker ().
作者信息
Zhang Xuan, Chi Hai, Peng Di, Jiang Mei, Wang Cuihua, Zhang Haiyan, Kang Wei, Li Lei
机构信息
East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China.
Key Laboratory of Protection and Utilization of Aquatic Germplasm Resource, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China.
出版信息
Foods. 2025 Mar 17;14(6):1016. doi: 10.3390/foods14061016.
Two typical fish products-fermented mandarin fish and semi-dried yellow croaker-are associated with biogenic amines (BAs), which are harmful to human health. The objective of this study was to investigate the bacterial ecology of the two fish products and to determine their capacity for producing BAs. Putrescine and cadaverine were major BAs detected in the fish products. Concentrations of BAs were significantly corrected with microbial count ( < 0.05). BA-producing isolates (33) in the two fish products were all multiple BA producers. Several of them, including , and isolated from fermented mandarin fish, as well as , , and isolated from semi-dried yellow croaker, showed remarkable BA-producing capacity. produced the greatest abundance of putrescine, cadaverine, tyramine and 2-phenylethylamine. mainly produced tryptamine and putrescine. showed the strongest histamine-producing capacity.
两种典型的鱼制品——糟鱼和半干黄鱼——与生物胺（BAs）有关，生物胺对人体健康有害。本研究的目的是调查这两种鱼制品的细菌生态学，并确定它们产生生物胺的能力。腐胺和尸胺是在鱼制品中检测到的主要生物胺。生物胺浓度与微生物数量显著相关（<0.05）。两种鱼制品中产生生物胺的分离株（33株）均为多种生物胺产生菌。其中一些菌株，包括从糟鱼中分离出的、和，以及从半干黄鱼中分离出的、、和，表现出显著的生物胺产生能力。产生的腐胺、尸胺、酪胺和2-苯乙胺含量最高。主要产生色胺和腐胺。表现出最强的组胺产生能力。
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