Marine College, Shandong University, Weihai 264209, China.
State Key Laboratory of Ballast Water Research, Comprehensive Technical Service Center of Jiangyin Customs, Jiangyin 214440, China.
Mar Pollut Bull. 2021 Aug;169:112523. doi: 10.1016/j.marpolbul.2021.112523. Epub 2021 May 25.
Despite that the ballast water management (BWM) convention has come into force to prevent the spread of harmful aquatic organisms, to date, very few bacteria can be identified through microbial culture method. In this study, we explored a reduced-representation sequencing of 2b-RAD approach to investigate the bacterial diversity in ballast water and sediments (BWS). Our results indicated a large amount of bacteria species (1496) detected in BWS up to now, including 13 pathogens that are seriously concerning in marine environment and aquaculture like the most harmful Vibrio harveyi and Aurantimonas coralicida. We showed that the ballast water had relative lower species, which was dominated by Proteobacteria. In contrast, the sediments had richer species, which was dominated by Bacteroidetes. Although BWS differed significantly in species composition, sediments shared most of the concerned pathogens with ballast water, highlighting the importance of sediment management. In conclusion, 2b-RAD sequencing shows promise in future BWM.
尽管压载水管理（BWM）公约已经生效，以防止有害水生生物的传播，但迄今为止，通过微生物培养方法只能识别出很少的细菌。在本研究中，我们探索了 2b-RAD 方法的简化代表性测序，以调查压载水和沉积物（BWS）中的细菌多样性。我们的结果表明，迄今为止，BWS 中检测到了大量的细菌物种（1496 种），其中包括 13 种在海洋环境和水产养殖中严重关注的病原体，如最具危害性的 Harveyi 弧菌和珊瑚 Aurantimonas 菌。我们表明，压载水中的物种相对较少，主要由变形菌门组成。相比之下，沉积物中的物种更丰富，主要由拟杆菌门组成。尽管 BWS 在物种组成上有很大的差异，但沉积物与压载水共享了大多数关注的病原体，这突出了沉积物管理的重要性。总之，2b-RAD 测序在未来的 BWM 中具有广阔的应用前景。
