College of Ocean Science and Engineering, Shanghai Maritime University, Shanghai 201306, China.
Jiangyin Customs, Jiangyin 214400, China.
Mar Pollut Bull. 2023 Sep;194(Pt B):115336. doi: 10.1016/j.marpolbul.2023.115336. Epub 2023 Aug 3.
Accurate detecting bacterial communities in ballast water and sediments supports risk management. This study uses full-length 16S rRNA gene sequencing to investigate the bacterial communities in ballast water and sediments, focusing on detecting pathogens. The results indicate that full-length sequencing more accurately reveals the species diversity. There is a significant difference (P < 0.05) in bacterial communities between ballast water and sediments, despite both being dominated by the Proteobacteria phylum. Thirty human and fish pathogens were identified by full-length sequencing, yet only five pathogens were detected from V3-V4 sequencing. Notably, emerging pathogens such as Citrobacter freundii and Nocardia nova are detected in samples, which are harmful to aquaculture and human health. Several opportunistic pathogens were also identified. In summary, this study provides important insights into the bacterial communities in ballast water and sediments, highlighting the need for strict management.
准确检测压载水和沉积物中的细菌群落有助于风险管理。本研究使用全长 16S rRNA 基因测序来调查压载水和沉积物中的细菌群落，重点是检测病原体。结果表明，全长测序更准确地揭示了物种多样性。尽管压载水和沉积物都以变形菌门为主，但它们的细菌群落存在显著差异（P<0.05）。全长测序鉴定出 30 个人类和鱼类病原体，但仅从 V3-V4 测序中检测到 5 种病原体。值得注意的是，在样本中检测到了一些新兴病原体，如弗氏柠檬酸杆菌和新诺卡氏菌，这些病原体对水产养殖和人类健康有害。还鉴定出了一些机会性病原体。总之，本研究深入了解了压载水和沉积物中的细菌群落，强调了严格管理的必要性。
