Barnea E R, MacLusky N J, Purdy R, Naftolin F
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT.
J Steroid Biochem. 1988 Aug;31(2):253-5. doi: 10.1016/0022-4731(88)90064-7.
Placental estrogen hydroxylase (EH) enzyme activity was measured at term using the catechol-O-methyl transferase coupled method in normal and high risk conditions. The identity and ratio of products formed during incubation of microsomes as analysed by high performance liquid chromatography in chronic hypertension, toxemia and diabetes mellitus was not different from controls. The mean enzymatic activity was also not different among the conditions studied as expressed mean +/- SE pmol/min/mg, protein: chronic hypertension (7.8 +/- 1), toxemia (8 +/- 1.6), diabetes mellitus (6.1 +/- 0.9) and controls (8.3 +/- 1.5). The cofactor dependence of EH was studied showing that NADPH is a better substrate for the enzyme than NADH.
采用儿茶酚-O-甲基转移酶偶联法，在足月时测量正常及高危情况下胎盘雌激素羟化酶（EH）的酶活性。通过高效液相色谱分析，慢性高血压、先兆子痫和糖尿病患者微粒体孵育过程中形成的产物的种类和比例与对照组并无差异。在所研究的不同情况下，以平均±标准误pmol/分钟/毫克蛋白质表示的平均酶活性也无差异：慢性高血压（7.8±1）、先兆子痫（8±1.6）、糖尿病（6.1±0.9）和对照组（8.3±1.5）。对EH的辅因子依赖性进行了研究，结果表明NADPH作为该酶的底物比NADH更佳。
