Rickard M D, Mackinlay L M, Kane G J, Matossian R M, Smyth J D
J Helminthol. 1977 Sep;51(3):221-8. doi: 10.1017/s0022149x00007562.
Brood capsules were obtained from freshly collected cysts of equine and ovine strains of Echinococcus granulosus. Protoscoleces were freed from brood capsules either by mechanical disruption or pepsin-HCI digestion. Preparations of protoscoleces studied included: mechanically released protoscoleces without further treatment, or incubated either in HCI pH 2.0 or in evaginating solution (containing Na taurocholate) for 24 h; pepsin-HCI released protoscoleces without further treatment or incubated in evaginating solution for 24 h or 7 days. Half of each preparation of ovine protoscoleces was fixed in absolute methanol. All fresh preparations of protoscoleces lysed rapidly when incubated in normal human serum. Studies with a fluorescein isothiocyanate (FITC) labelled sheep anti-human C3 antiserum revealed the presence of C3 on the surface of lysing protoscoleces. Antibody could not be detected on the surface of any of the preparations of fresh or methanol-fixed protoscoleces using direct or indirect fluorescent antibody tests suggesting that the classical pathway of complement activation was not involved in the lytic process. Strong evidence for lysis by the alternate pathway of complement activation was the lysis of protoscoleces which had been treated with pepsin-HCI and lysis of protoscoleces in guinea-pig serum deficient in C4 component of complement.
包囊蚴是从新鲜采集的细粒棘球绦虫马和羊品系的囊中获得的。原头蚴通过机械破碎或胃蛋白酶 - 盐酸消化从包囊蚴中释放出来。所研究的原头蚴制剂包括：未经进一步处理的机械释放的原头蚴，或在pH 2.0的盐酸中或在外翻溶液（含牛磺胆酸钠）中孵育24小时；胃蛋白酶 - 盐酸释放的原头蚴，未经进一步处理或在外翻溶液中孵育24小时或7天。羊原头蚴每种制剂的一半用无水甲醇固定。所有新鲜的原头蚴制剂在正常人血清中孵育时迅速裂解。用异硫氰酸荧光素（FITC）标记的羊抗人C3抗血清进行的研究表明，裂解的原头蚴表面存在C3。使用直接或间接荧光抗体试验，在新鲜或甲醇固定的原头蚴的任何制剂表面均未检测到抗体，这表明补体激活的经典途径未参与裂解过程。补体激活替代途径导致裂解的有力证据是，经胃蛋白酶 - 盐酸处理的原头蚴的裂解以及在缺乏补体C4成分的豚鼠血清中原头蚴的裂解。
