使用一步荧光探针进行烟酰胺腺嘌呤二核苷酸依赖的去乙酰化酶活性的活细胞成像。
Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe.
机构信息
Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Aichi, Japan.
出版信息
Methods Mol Biol. 2021;2274:155-168. doi: 10.1007/978-1-0716-1258-3_14.
Sirtuins (SIRTs) are a family of NAD-dependent histone deacetylases (HDACs). In mammals, dysfunction of SIRTs is associated with age-related metabolic diseases, cancers, and even aging. Therefore, the detection of SIRT activity in living cells or tissues would be helpful for diagnosis of a wide range of SIRT-associated diseases. Here, we present methodology to measure SIRT activity in living cells by using our newly developed SIRT fluorescence probe, KST-F-DA.
Sirtuins (SIRTs) 是一类 NAD 依赖的组蛋白去乙酰化酶 (HDACs)。在哺乳动物中，SIRTs 的功能障碍与年龄相关的代谢疾病、癌症，甚至衰老有关。因此，在活细胞或组织中检测 SIRT 的活性将有助于诊断广泛的 SIRT 相关疾病。在这里，我们通过使用我们新开发的 SIRT 荧光探针 KST-F-DA，提出了一种在活细胞中测量 SIRT 活性的方法。
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