Picaud Sandrine, Lambolez Bertrand, Tricoire Ludovic
Neuroscience Paris Seine-Institut de Biologie Paris Seine (NPS-IBPS), Sorbonne Université UM119, CNRS UMR8246, INSERM U1130, Paris, France.
Methods Mol Biol. 2021;2274:281-294. doi: 10.1007/978-1-0716-1258-3_24.
Optogenetic calcium sensors enable the imaging in real-time of the activities of single or multiple neurons in brain slices and in vivo. Bioluminescent probes engineered from the natural calcium sensor aequorin do not require illumination, are virtually devoid of background signal, and exhibit wide dynamic range and low cytotoxicity. These probes are thus well suited for long-duration, whole-field recordings of multiple neurons simultaneously. Here, we describe a protocol for monitoring and analyzing the dynamics of neuronal ensembles using whole-field bioluminescence imaging of an aequorin-based sensor in brain slice.
光遗传学钙传感器能够实时成像脑片和体内单个或多个神经元的活动。由天然钙传感器水母发光蛋白改造而来的生物发光探针不需要光照，几乎没有背景信号，具有宽动态范围和低细胞毒性。因此，这些探针非常适合同时对多个神经元进行长时间的全场记录。在这里，我们描述了一种使用基于水母发光蛋白的传感器在脑片中进行全场生物发光成像来监测和分析神经元集群动态的方法。
