China Agricultural Vet. Bio. Science and Technology Co., Ltd., Gansu, 730046, Lanzhou, China.
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.
Parasitol Res. 2021 Jun;120(6):2297-2301. doi: 10.1007/s00436-020-06792-3. Epub 2021 May 29.
In order to find a new preservation solution for avian coccidial oocysts that can replace potassium dichromate (KCrO) solution, Eimeria tenella oocysts were preserved in 0.1 to 10% potassium sorbate (CHKO) solution in this study. The results showed that there was no significant difference between the sporulation rate of E. tenella oocysts preserved in 0.1 to 10% CHKO solution and in 2.5% KCrO solution (p > 0.05). The 0.5 to 10% CHKO solution could also effectively inhibit the growth of bacterial microorganisms. E. tenella oocysts preserved in 1% CHKO solution at 4 °C for 3, 6, 9, and 12 months, with the oocyst production of E. tenella oocysts being 1.3-, 1.2-, 1.6-, and 1.3-fold higher than that of oocysts stored in 2.5% KCrO solution (p < 0.05). In conclusion, CHKO could replace KCrO as the preservation solution of avian coccidial oocysts.
为了寻找一种可替代重铬酸钾（KCrO）溶液的禽类球虫孢子保存新方法，本研究采用 0.1%至 10%山梨酸钾（CHKO）溶液保存柔嫩艾美耳球虫孢子。结果表明，0.1%至 10% CHKO 溶液保存柔嫩艾美耳球虫孢子的孢子化率与 2.5% KCrO 溶液之间无显著差异（p＞0.05）。0.5%至 10% CHKO 溶液也能有效抑制细菌微生物的生长。在 4℃下，1% CHKO 溶液可将柔嫩艾美耳球虫孢子的保存时间延长至 3、6、9 和 12 个月，与在 2.5% KCrO 溶液中保存的孢子相比，球虫孢子产量分别提高了 1.3 倍、1.2 倍、1.6 倍和 1.3 倍（p＜0.05）。综上所述，CHKO 可替代 KCrO 作为禽类球虫孢子的保存溶液。
