Department of Physiology and Pathophysiology, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, R3E 0T5, Canada; Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Albrechtsen Research Centre, Winnipeg, MB, R2H 2A6, Canada.
Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Albrechtsen Research Centre, Winnipeg, MB, R2H 2A6, Canada; Department of Food and Human Nutritional Sciences, Faculty of Agricultural and Food Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
Prostaglandins Leukot Essent Fatty Acids. 2021 Jul;170:102284. doi: 10.1016/j.plefa.2021.102284. Epub 2021 Apr 20.
Consumption of omega-3 polyunsaturated fatty acids (n-3 PUFAs) has been reported to provide health benefits, but it remains unknown whether the fatty acids themselves or their oxygenated metabolites, oxylipins, are responsible for the beneficial effects.
This paper describes the design and rationale of a randomized, double-blinded, cross-over study comparing the effects of α-linolenic acid (ALA)-rich flax oil and docosahexaenoic acid (DHA)-rich fish oil supplementation on circulating oxylipin profiles in females with obesity, in relation to obesity-induced inflammation.
Pre-menopausal females (n = 24) aged 20-55 with a BMI ≥30, will consume capsules containing flaxseed oil (4 g ALA/day) or fish oil (4 g DHA + 0.8 g EPA/day) during 4-week supplementation phases, with a minimum 4-week washout. The primary outcome is alterations in plasma oxylipin profiles. Secondary outcomes include effects of supplementation on circulating markers of inflammation, adipokines, plasma fatty acid composition, blood lipid profile, anthropometrics, oxylipin and cytokine profiles of stimulated immune cells, monocyte glucose metabolism, blood pressure and pulse wave velocity.
This trial has been approved by the University of Manitoba Biomedical Research Ethics Board and the St. Boniface Hospital Research Review Committee. The study will provide information regarding the effects of ALA and DHA supplementation on oxylipin profiles in obese but otherwise healthy females. Additionally, it will improve our understanding of the response of circulating inflammatory mediators originating from immune cells, adipose tissue and the liver to n-3 PUFA supplementation in relation to the metabolic features of obesity.
已报道摄入ω-3 多不饱和脂肪酸（n-3PUFAs）可带来健康益处，但尚不清楚是脂肪酸本身还是其氧化代谢物，即类二十烷酸，对这些有益作用负责。
本文介绍了一项随机、双盲、交叉研究的设计和原理，该研究比较了富含α-亚麻酸（ALA）的亚麻籽油和富含二十二碳六烯酸（DHA）的鱼油补充剂对肥胖女性循环类二十烷酸谱的影响，与肥胖引起的炎症有关。
将 20-55 岁、BMI≥30 的绝经前女性（n=24）分为两组，分别摄入亚麻籽油胶囊（每天 4 g ALA）或鱼油胶囊（每天 4 g DHA+0.8 g EPA），进行为期 4 周的补充阶段，洗脱期至少 4 周。主要结局是改变血浆类二十烷酸谱。次要结局包括补充对循环炎症标志物、脂肪因子、血浆脂肪酸组成、血脂谱、人体测量学、刺激免疫细胞中环氧化合物和细胞因子谱、单核细胞葡萄糖代谢、血压和脉搏波速度的影响。
该试验已获得马尼托巴大学生物医学研究伦理委员会和圣博尼费斯医院研究审查委员会的批准。该研究将提供有关 ALA 和 DHA 补充对肥胖但其他方面健康的女性类二十烷酸谱影响的信息。此外，它将增进我们对循环炎症介质（源自免疫细胞、脂肪组织和肝脏）对 n-3PUFA 补充的反应与肥胖的代谢特征之间的关系的理解。
