State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237, China; Key Laboratory of Bio-based Material Engineering of China National Light Industry Council, 130 Meilong Road, Shanghai 200237, China.
Bioresour Technol. 2021 Sep;336:125323. doi: 10.1016/j.biortech.2021.125323. Epub 2021 May 24.
Acetate is a potential non-food carbon source for industrial production, coping with the shortage of food-based feedstocks. (R)-3-hydroxybutyric acid (R-3HB) can be used as an important chiral intermediate in the fine chemical and pharmaceutical industry. In this study, the R-3HB biosynthesis pathway was successfully constructed when genes of β-ketothiolase (phaA), acetoacetyl-CoA reductase (phaB) from Ralstonia eutropha, and propionyl-CoA transferases (pct) from Clostridium beijerinckii 8052 were introducedinto Escherichia coli. The effects of host E. coli strains, different propionyl-CoA transferases, and post-induction temperatures were investigated. The final concentration of R-3HB reached 0.86 g/L using acetate as the sole carbon source. Subsequently, a kind of culture broth containing the syngas-derived acetate was used to produce 1.02 g/L of R-3HB with a yield of 0.26 g/g. Inthis study, the engineered E. coli strain could efficiently utilize syngas-derived acetate to synthesize R-3HB.
醋酸盐是一种潜在的非食品碳源，可用于工业生产，以应对基于食物的原料短缺问题。（R）-3-羟基丁酸（R-3HB）可用作精细化工和制药行业的重要手性中间体。在本研究中，当引入来自 Ralstonia eutropha 的β-酮硫解酶（phaA）、乙酰乙酰辅酶 A 还原酶（phaB）和来自 Clostridium beijerinckii 8052 的丙酰辅酶 A 转移酶（pct）的基因时，成功构建了 R-3HB 生物合成途径。研究了宿主大肠杆菌菌株、不同的丙酰辅酶 A 转移酶和诱导后温度对 R-3HB 产量的影响。当以醋酸盐为唯一碳源时，R-3HB 的最终浓度达到 0.86 g/L。随后，利用含有合成气衍生醋酸盐的一种培养基于 1.02 g/L 的产率合成了 0.26 g/g 的 R-3HB。在本研究中，工程大肠杆菌菌株可以有效地利用合成气衍生醋酸盐来合成 R-3HB。
