Institute of Medical Photonics, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.
Institute of Clinical Pharmacology, Peking University First Hospital, Beijing, 100034, China.
Anal Chim Acta. 2021 Jul 11;1168:338622. doi: 10.1016/j.aca.2021.338622. Epub 2021 May 7.
Methods for rapid antimicrobial susceptibility testing (AST) are urgently needed to address the emergence and spread of antimicrobial resistance. Here, we report a new method based on stimulated Raman scattering (SRS) microscopy, which measures both the metabolic activity and the morphological deformation of bacteria to determine the antimicrobial susceptibility of β-lactam antibiotics rapidly. In this approach, we quantify single bacteria's metabolic activity by the carbon-deuterium (C-D) bond concentrations in bacteria after DO incubation. In the meantime, bacterial morphological deformation caused by β-lactam antibiotics is also measured. With these two quantifiable markers, we develop an evaluation method to perform AST of cefotaxime on 103 E. coli strains. Our method achieved a 93.2% categorical agreement and a 93.2% essential agreement with the standard reference method.
方法快速抗菌药敏试验（AST）是迫切需要解决的出现和传播的抗菌药物耐药性。在这里，我们报告了一种新的方法，基于受激拉曼散射（SRS）显微镜，它可以同时测量细菌的代谢活性和形态变形来快速确定β-内酰胺类抗生素的药敏性。在这种方法中，我们通过 DO 孵育后细菌中的碳氘（C-D）键浓度来量化单个细菌的代谢活性。同时，也测量了β-内酰胺类抗生素引起的细菌形态变形。有了这两个可量化的标记物，我们开发了一种评估方法，对 103 株大肠杆菌进行头孢噻肟的 AST。我们的方法与标准参考方法的分类符合率为 93.2%，实质符合率为 93.2%。
