Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, 100081, China.
Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, 100191, China.
Toxicon. 2021 Aug;199:31-40. doi: 10.1016/j.toxicon.2021.05.011. Epub 2021 May 28.
Botulinum toxin type A (BTXA) is effective for the treatment of sialorrhea. MicroRNAs (miRNAs) have significant functions in salivary diseases, but the role of miRNAs during BTXA-inhibited salivary secretion is not yet clear. A total of 19 differentially expressed (DE) miRNAs and 1072 DE mRNAs were identified following BTXA injected into submandibular glands of rats (n = 4) through miRNA sequencing and microarray analysis. Bioinformatic analysis identified that several pathways may be associated with the inhibition of salivary secretion, such as the MAPK signalling pathway, tight junctions, and cytokine-cytokine receptor interaction. We predicted the target genes of DE miRNAs and established the miRNA-mRNA interaction network. The intersection of DE mRNAs and target genes of DE miRNAs was performed and seven mRNAs were obtained: Egr2, Paqr9, Zkscan1, Usp6n, Cyb561a3, Zfhx4, and Clic5. These findings explore the mechanism of BTXA in inhibiting salivary secretion and probably will provide new ideas for clinical application.
A型肉毒毒素（BTXA）对治疗流涎症有效。微小 RNA（miRNA）在唾液疾病中具有重要功能，但 miRNA 在 BTXA 抑制唾液分泌中的作用尚不清楚。通过 miRNA 测序和微阵列分析，在向大鼠颌下腺注射 BTXA 后（n=4），共鉴定出 19 个差异表达（DE）miRNA 和 1072 个 DEmRNA。生物信息学分析表明，几个途径可能与唾液分泌抑制有关，如 MAPK 信号通路、紧密连接和细胞因子-细胞因子受体相互作用。我们预测了 DEmiRNA 的靶基因，并建立了 miRNA-mRNA 相互作用网络。对 DEmRNA 和 DEmiRNA 靶基因的交集进行了分析，得到了 7 个 mRNA：Egr2、Paqr9、Zkscan1、Usp6n、Cyb561a3、Zfhx4 和 Clic5。这些发现探索了 BTXA 抑制唾液分泌的机制，可能为临床应用提供新的思路。
