Mielicki W, Wierzbicki R
Department of Biochemistry, Institute of Environmental Research and Bioanalysis, Medical Academy, Lódz, Poland.
Neoplasma. 1988;35(3):279-84.
The procoagulant activity (PCA) was examined in extracts from primary tumor and metastases of experimental Guerin epithelioma transplanted into Wistar rats. PCA was evaluated by measurement of the level of activation of coagulation factor X using specific chromogenic substrate S-2222. We observed that extracts from both primary tumor and metastases were able to activate factor X in vitro. This activity was not factor VII-dependent, it was inhibited by phenylmethylsulfonyl fluoride (PMSF) and mercuric chloride, and in a smaller degree by p-chloromercuric benzoate (p-CMB) and iodoacetic acid (IAA). No difference between procoagulant activity in extracts from primary tumor and from metastases was observed.
对移植到Wistar大鼠体内的实验性格伦上皮瘤原发肿瘤和转移灶提取物中的促凝活性（PCA）进行了检测。通过使用特异性显色底物S-2222测量凝血因子X的活化水平来评估PCA。我们观察到，原发肿瘤和转移灶的提取物在体外均能激活因子X。该活性不依赖于因子VII，它被苯甲基磺酰氟（PMSF）和氯化汞抑制，在较小程度上被对氯汞苯甲酸（p-CMB）和碘乙酸（IAA）抑制。未观察到原发肿瘤提取物和转移灶提取物的促凝活性之间存在差异。
