Zucchella M, Pacchiarini L, Tacconi F, Saporiti A, Grignani G
Department of Internal Medicine, University of Pavia, Italy.
Thromb Haemost. 1993 Apr 1;69(4):335-8.
We studied in a homologous system the procoagulant activity of human tumor cells cultured "in vitro" (1402 primary melanoma, Me 7110/2 metastatic melanoma, Hep G2 hepatoma and GLC1 small cell lung carcinoma) or of cells freshly isolated from different human tumor tissues. Tumor cells cultured "in vitro" possessed and released a factor VII dependent procoagulant activity, which was inhibited by concanavalin A and unaffected by iodoacetamide or HgCl2. The activity released by the cells of metastatic melanoma was higher than that released by the cells of the primary tumor. On the contrary, cancer cells isolated from tumor tissues possessed and released a factor VII independent activity which was inhibited by iodoacetamide of HgCl2 and was not modified by concanavalin A. Therefore, different methods for the preparation of tumor cell suspensions have to be used for the study of tumor procoagulants, since their expression depends very largely on the source of tumor cells. Furthermore, cultured human tumor cells are not an appropriate model for the "in vivo" procoagulant effect of tumor cells.
我们在同源系统中研究了体外培养的人肿瘤细胞（1402原发性黑色素瘤、Me 7110/2转移性黑色素瘤、Hep G2肝癌和GLC1小细胞肺癌）或从不同人肿瘤组织中新鲜分离的细胞的促凝活性。体外培养的肿瘤细胞具有并释放一种依赖因子VII的促凝活性，该活性被伴刀豆球蛋白A抑制，不受碘乙酰胺或HgCl2影响。转移性黑色素瘤细胞释放的活性高于原发性肿瘤细胞释放的活性。相反，从肿瘤组织分离的癌细胞具有并释放一种不依赖因子VII的活性，该活性被碘乙酰胺或HgCl2抑制，且不受伴刀豆球蛋白A影响。因此，由于肿瘤促凝剂的表达在很大程度上取决于肿瘤细胞的来源，所以必须使用不同的方法制备肿瘤细胞悬液来研究肿瘤促凝剂。此外，培养的人肿瘤细胞不是研究肿瘤细胞体内促凝作用的合适模型。
