Nicola Federico, Azula Natalia, Santoni Gabriela, Smayevsky Jorgelina
Laboratorio de Bacteriología, Micología y Parasitología; Departamento de Análisis Clínicos; Centro de Educación Médica e Investigaciones Clínicas (CEMIC), Av. Galván 4102, Ciudad Autónoma de Buenos Aires, Argentina.
Laboratorio de Bacteriología, Micología y Parasitología; Departamento de Análisis Clínicos; Centro de Educación Médica e Investigaciones Clínicas (CEMIC), Av. Galván 4102, Ciudad Autónoma de Buenos Aires, Argentina.
Rev Argent Microbiol. 2022 Apr-Jun;54(2):114-119. doi: 10.1016/j.ram.2021.02.012. Epub 2021 May 28.
In vitro activities of delafloxacin, ciprofloxacin and levofloxacin were evaluated by epsilometric and disk diffusion methods against 181 bacterial isolates recovered from bone and skin infections. Isolates included were 84 Staphylococcus aureus (40 MRSA and 44 MSSA), 46 coagulase-negative staphylococci (CNS), 23 Klebsiella pneumoniae and 28 Pseudomonas aeruginosa. The MIC/MIC (mg/l) for delafloxacin, ciprofloxacin and levofloxacin, respectively, were: MRSA, 0.004/0.064, 0.25/16 and 0.125/4; MSSA, 0.002/0.004, 0.125/0.25 and 0.125/0.25; CNS, 0.008/0.25, 0.125/>32 and 0.25/>32; K. pneumoniae, 4/>32,>32/>32 and 16/>32; P. aeruginosa, 1/>32, 0,5/>32 and 4/>32. Susceptibilities for delafloxacin, ciprofloxacin and levofloxacin, respectively, were: MRSA, 97.5%, 82.5% and 82.5%; MSSA, 97.7%, 95.5% and 95.5%; CNS, 93.5%, 63.0% and 60.9%; K. pneumoniae, 21.7%, 26.1% and 43.5%; P aeruginosa, 35.7%, 53.6% and 42.8%. The disk diffusion and epsilometric methods were concordant for evaluating in vitro susceptibility in staphylococci (categorical concordance of 98.8% for S. aureus and 91.3% for CNS).
采用比浊法和纸片扩散法，对从骨感染和皮肤感染中分离出的181株细菌，评估了德拉氟沙星、环丙沙星和左氧氟沙星的体外活性。分离出的细菌包括84株金黄色葡萄球菌（40株耐甲氧西林金黄色葡萄球菌和44株甲氧西林敏感金黄色葡萄球菌）、46株凝固酶阴性葡萄球菌（CNS）、23株肺炎克雷伯菌和28株铜绿假单胞菌。德拉氟沙星、环丙沙星和左氧氟沙星的MIC/MIC（mg/l）分别为：耐甲氧西林金黄色葡萄球菌，0.004/0.064、0.25/16和0.125/4；甲氧西林敏感金黄色葡萄球菌，0.002/0.004、0.125/0.25和0.125/0.25；凝固酶阴性葡萄球菌，0.008/0.25、0.125/>32和0.25/>32；肺炎克雷伯菌，4/>32、>32/>32和16/>32；铜绿假单胞菌，1/>32、0.5/>32和4/>32。德拉氟沙星、环丙沙星和左氧氟沙星的敏感性分别为：耐甲氧西林金黄色葡萄球菌，97.5%、82.5%和82.5%；甲氧西林敏感金黄色葡萄球菌，97.7%、95.5%和95.5%；凝固酶阴性葡萄球菌，93.5%、63.0%和60.9%；肺炎克雷伯菌，21.7%、26.1%和43.5%；铜绿假单胞菌，35.7%、53.6%和42.8%。纸片扩散法和比浊法在评估葡萄球菌的体外敏感性方面具有一致性（金黄色葡萄球菌的分类一致性为98.8%，凝固酶阴性葡萄球菌为91.3%）。
