Yang Bo, Zhao Fang, Yao Lei, Zong Zhenfeng, Xiao Li
Department of Thoracic Surgery, Cangzhou Central Hospital, Hebei, China.
Department of Hematology, Cangzhou Central Hospital, Hebei, China.
Front Pharmacol. 2021 May 13;12:657053. doi: 10.3389/fphar.2021.657053. eCollection 2021.
Circular RNAs (circRNAs) have been demonstrated in playing an important role in the physiological and pathological processes (such as cancer). This paper aims to clarify the role of Circ_0006677 in non-small-cell lung cancer (NSCLC) progression. Using clinical data and cell line models, we revealed the tumor-suppressive role of circ_0006677 in lung cancer. Using the online bioinformatics tool, we predicted the target of circ_0006677 and further validated its regulatory mechanisms responsible for its tumor suppressor function in NSCLC. Circ_0006677 expression was reduced in NSCLC tissues of patients and lung cancer cells in comparison to adjacent normal tissues. Lower expression of circ_0006677 was significantly associated with poorer patient survival. Overexpression of circ_0006677 significantly inhibited the ability of NSCLC cell proliferation, migration, invasion, and glycolysis. Mechanically, circ_0006677 could inhibit NSCLC progression and glycolysis by regulating the expression of the signal transducer inhibitor SOSC2 through sponging microRNA-578 (miR-578). Circ_0006677 prevents the progression of NSCLC modulating the miR-578/SOSC2 axis.
环状RNA（circRNAs）已被证明在生理和病理过程（如癌症）中发挥重要作用。本文旨在阐明Circ_0006677在非小细胞肺癌（NSCLC）进展中的作用。利用临床数据和细胞系模型，我们揭示了circ_0006677在肺癌中的肿瘤抑制作用。使用在线生物信息学工具，我们预测了circ_0006677的靶标，并进一步验证了其在NSCLC中发挥肿瘤抑制功能的调控机制。与相邻正常组织相比，NSCLC患者组织和肺癌细胞中circ_0006677的表达降低。circ_0006677的低表达与患者较差的生存率显著相关。circ_0006677的过表达显著抑制了NSCLC细胞的增殖、迁移、侵袭和糖酵解能力。机制上，circ_0006677可通过海绵化微小RNA-578（miR-578）调节信号转导抑制剂SOSC2的表达，从而抑制NSCLC进展和糖酵解。circ_0006677通过调节miR-578/SOSC2轴来阻止NSCLC的进展。
