Li Chan, Fu Qingxia, Cai Jin, Mei Hongxia, Shangguan Wangning
Department of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
Key Laboratory of Anesthesiology of Zhejiang Province, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
Exp Ther Med. 2021 Jul;22(1):733. doi: 10.3892/etm.2021.10165. Epub 2021 May 9.
Liver cancer is a malignant cancer with worldwide prevalence. It has been reported that cancer cells are usually exposed to a hypoxic microenvironment, which is associated with a poor prognosis in patients with cancer. Propofol is an intravenous anesthetic that is widely used in cancer surgery. The present study aimed to determine the effects of propofol stimulation on the viability, proliferation and migration of liver cancer cells under normoxia and cobalt chloride (CoCl)-induced hypoxia. Under normoxia, HepG2 and HCCLM3 cells were randomly divided into six groups as follows: i) Control group; ii) 10 µM propofol group; iii) 25 µM propofol group; iv) 50 µM propofol group; v) 100 µM propofol group; and vi) DMSO group. Cell viability and proliferation were analyzed using Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) assays, respectively, following 24 or 48 h of propofol treatment. In addition, wound healing and Transwell migration assays were used to determine the changes in cell migration. Under CoCl-induced hypoxia, the protein levels of hypoxia inducible factor-1α (HIF-1α) of HepG2 cells were analyzed using western blotting. Subsequently, CCK-8 and wound healing assays were used to determine the effect of propofol on cell viability and migration. The results of the present study revealed that propofol stimulation had no significant effect on the viability, proliferation and migration of HepG2 and HCCLM3 cells under normoxia. The protein levels of HIF-1α were significantly upregulated following the treatment with 200 µM CoCl for 12 h. However, no significant differences were found in the viability and migration of HepG2 cells following the stimulation with propofol in the presence of CoCl. In conclusion, the findings of the present study revealed that propofol exerted no effect on the viability, proliferation and migration of HepG2 and HCCLM3 cells under normoxic and hypoxic conditions.
肝癌是一种在全球范围内普遍存在的恶性肿瘤。据报道，癌细胞通常处于缺氧微环境中，这与癌症患者的不良预后相关。丙泊酚是一种广泛用于癌症手术的静脉麻醉剂。本研究旨在确定在常氧和氯化钴（CoCl）诱导的缺氧条件下，丙泊酚刺激对肝癌细胞活力、增殖和迁移的影响。在常氧条件下，将HepG2和HCCLM3细胞随机分为六组，如下：i）对照组；ii）10 μM丙泊酚组；iii）25 μM丙泊酚组；iv）50 μM丙泊酚组；v）100 μM丙泊酚组；vi）二甲基亚砜（DMSO）组。在丙泊酚处理24或48小时后，分别使用细胞计数试剂盒-8（CCK-8）和5-乙炔基-2'-脱氧尿苷（EdU）检测分析细胞活力和增殖。此外，使用伤口愈合和Transwell迁移检测来确定细胞迁移的变化。在CoCl诱导的缺氧条件下，使用蛋白质印迹法分析HepG2细胞中缺氧诱导因子-1α（HIF-1α）的蛋白质水平。随后，使用CCK-8和伤口愈合检测来确定丙泊酚对细胞活力和迁移的影响。本研究结果表明，在常氧条件下，丙泊酚刺激对HepG2和HCCLM3细胞的活力、增殖和迁移没有显著影响。在用200 μM CoCl处理12小时后，HIF-1α的蛋白质水平显著上调。然而，在CoCl存在的情况下，用丙泊酚刺激后，HepG2细胞的活力和迁移没有发现显著差异。总之，本研究结果表明，在常氧和缺氧条件下，丙泊酚对HepG2和HCCLM3细胞的活力、增殖和迁移没有影响。
