Mao Hai-Xiang, Chen Bai-Wen, Wang Jie, Ma Chen-Yang, Gan Yi-Chao, Qiu Kai-Jie
Department of Pancreatic and Hepatobiliary Surgery, Ningbo Medical Treatment Center, Lihuili Hospital, Ningbo, Zhejiang 315000, P.R. China.
Exp Ther Med. 2021 Jul;22(1):780. doi: 10.3892/etm.2021.10212. Epub 2021 May 19.
MicroRNA (miRNA/miR)-3677 has been indicated to be negatively associated with the survival of patients with hepatocellular carcinoma (HCC) based on The Cancer Genome Atlas database. However, as a novel miRNA, the role of miR-3677-5p in HCC has remained to be elucidated. In the present study, the expression of miR-3677-5p was assessed in HCC tissues and cell lines using reverse transcription-quantitative PCR. Survival analysis was performed using Kaplan-Meier curves. Furthermore, the prognostic significance of miR-3677-5p was evaluated using Cox regression analysis. The effects of miR-3677-5p on cell proliferation, as well as migration and invasion capacities, were analyzed using Cell Counting Kit-8, crystal violet and Transwell assays. The results demonstrated that the level of miR-3677-5p expression was upregulated in human HCC tissues and cell lines and that miR-3677-5p expression was closely associated with tumor size, TNM stage and vascular invasion. Furthermore, high miR-3677-5p expression was significantly associated with unfavorable clinical prognosis for patients with HCC. Overexpression of miR-3677-5p was indicated to significantly promote the proliferation, migration and invasion of HCC cells, whereas knockdown of miR-3677-5p was observed to have an inhibitory effect. In conclusion, the present study demonstrated that miR-3677-5p acts as an oncogene that has a critical role in the regulation of HCC proliferation and progression. Hence, miR-3677-5p may serve as a valuable prognostic biomarker and may be developed as a promising therapeutic target for HCC.
基于癌症基因组图谱数据库，已表明微小RNA（miRNA/miR）-3677与肝细胞癌（HCC）患者的生存率呈负相关。然而，作为一种新型miRNA，miR-3677-5p在HCC中的作用仍有待阐明。在本研究中，使用逆转录定量PCR评估了miR-3677-5p在HCC组织和细胞系中的表达。使用Kaplan-Meier曲线进行生存分析。此外，使用Cox回归分析评估miR-3677-5p的预后意义。使用细胞计数试剂盒-8、结晶紫和Transwell实验分析了miR-3677-5p对细胞增殖以及迁移和侵袭能力的影响。结果表明，miR-3677-5p在人HCC组织和细胞系中的表达水平上调，且miR-3677-5p的表达与肿瘤大小、TNM分期和血管侵犯密切相关。此外，miR-3677-5p高表达与HCC患者不良的临床预后显著相关。miR-3677-5p的过表达被表明可显著促进HCC细胞的增殖、迁移和侵袭，而miR-3677-5p的敲低则具有抑制作用。总之，本研究表明miR-3677-5p作为一种癌基因，在HCC增殖和进展的调控中起关键作用。因此，miR-3677-5p可能作为一种有价值的预后生物标志物，并可能被开发为HCC有前景的治疗靶点。
