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通过生物信息学分析及在骨肉瘤细胞系中的验证鉴定KIF4A作为泛癌诊断和预后生物标志物

Identification of KIF4A as a pan-cancer diagnostic and prognostic biomarker via bioinformatics analysis and validation in osteosarcoma cell lines.

作者信息

Pan Jiankang, Lei Xiaohua, Mao Xinzhan

机构信息

Department of Orthopedics, the Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

Department of Hepato-Biliary-Pancreatic Surgery, the First Affiliated Hospital of University of South China, Hengyang, Hunan, China.

出版信息

PeerJ. 2021 May 21;9:e11455. doi: 10.7717/peerj.11455. eCollection 2021.

DOI:10.7717/peerj.11455
PMID:34055488
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8142929/
Abstract

BACKGROUND

Cancer is a disease of abnormal cell proliferation caused by abnormal expression of cancer-related genes. However, it is still difficult to distinguish benign and malignant lesions in many cases. KIF4A has been reported to be associated with a variety of cancer lesions. We aimed to explore whether KIF4A could be used as a biomarker of pan-cancer diagnostic.

METHODS

We identified twenty-eight cell cycle-related genes that were overexpressed in no less than ten types of cancer. We determined KIF4A mRNA and protein expression in osteosarcoma (OS) cells. Furthermore, to determine the effect of KIF4A in OS, we silenced KIF4A in OS cells and detected cell viability, colony formation, invasion, migration, apoptosis and cell cycle parameters.

RESULTS

KIF4A exhibited upregulated expression in eleven types of cancer. Cell cycle-related genes are extensively overexpressed in various types of cancers. KIF4A overexpression can serve as a diagnostic and prognostic marker in various cancers. Silencing KIF4A inhibited the viability, colony formation, invasion and migration and induced apoptosis and cell cycle arrest of OS cells. Our findings revealed that high expression of KIF4A could serve as a diagnostic and prognostic marker in OS cancers.

CONCLUSION

KIF4A could serve as a pan-cancer diagnostic and prognostic marker. KIF4A could be used as a novel therapeutic target for OS.

摘要

背景

癌症是一种由癌症相关基因异常表达引起的细胞异常增殖疾病。然而，在许多情况下，区分良性和恶性病变仍然很困难。据报道，KIF4A与多种癌症病变有关。我们旨在探讨KIF4A是否可作为泛癌诊断的生物标志物。

方法

我们鉴定了28个在不少于10种癌症类型中过表达的细胞周期相关基因。我们测定了骨肉瘤（OS）细胞中KIF4A的mRNA和蛋白质表达。此外，为了确定KIF4A在骨肉瘤中的作用，我们在骨肉瘤细胞中沉默了KIF4A，并检测了细胞活力、集落形成、侵袭、迁移、凋亡和细胞周期参数。

结果

KIF4A在11种癌症类型中表达上调。细胞周期相关基因在各种类型的癌症中广泛过表达。KIF4A过表达可作为各种癌症的诊断和预后标志物。沉默KIF4A可抑制骨肉瘤细胞的活力、集落形成、侵袭和迁移，并诱导其凋亡和细胞周期停滞。我们的研究结果表明，KIF4A的高表达可作为骨肉瘤的诊断和预后标志物。

结论

KIF4A可作为泛癌诊断和预后标志物。KIF4A可作为骨肉瘤的新型治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d3e/8142929/19570e005c2d/peerj-09-11455-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d3e/8142929/a47df106e723/peerj-09-11455-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d3e/8142929/4e1f60387a59/peerj-09-11455-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d3e/8142929/054b51af15ef/peerj-09-11455-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d3e/8142929/a46b04483726/peerj-09-11455-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d3e/8142929/19570e005c2d/peerj-09-11455-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d3e/8142929/a47df106e723/peerj-09-11455-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d3e/8142929/4e1f60387a59/peerj-09-11455-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d3e/8142929/054b51af15ef/peerj-09-11455-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d3e/8142929/a46b04483726/peerj-09-11455-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d3e/8142929/19570e005c2d/peerj-09-11455-g005.jpg

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2
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J Pers Med. 2020 Sep 15;10(3):128. doi: 10.3390/jpm10030128.
3
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4
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