College of Life Sciences, Hefei Normal University, Hefei 230601, People's Republic of China.
J Genet. 2021;100.
has recently emerged as an important bioenergy plant which is an ideal alternative for fossil fuels. It is particularly significant to analyse the codon usage bias (CUB) and further evaluate the intraspecific genetic divergence of three in Asia, considering its potential economic benefits and various utilities. In the present study, the patterns of CUB were systematically compared, and the factors shaping CUB were identified in all three genomes of . Our observations indicate that the preference for A/T nucleotides and A/T ending codons was present in all the three genomes. Moreover, 11 identical high-frequency codons as well as the optimal expression receptor were confirmed. Besides, it was observed that CUB resulted from the combined effects of natural selection and mutation pressure, while the natural selection was the determining factor. Eventually, similarity indices based on relative synonymous codon usage (RSCU) values implied low intraspecific genetic divergence in three Asian . This study provides useful clues for improving the expression level of exogenous genes and optimizing breeding programmes by molecular-assisted breeding in .
最近出现了一种重要的生物能源植物，它是化石燃料的理想替代品。分析亚洲三个物种的密码子使用偏性（CUB）并进一步评估种内遗传差异具有重要意义，因为它具有潜在的经济效益和多种用途。在本研究中，系统比较了 CUB 模式，并确定了三个亚洲物种基因组中 CUB 形成的因素。我们的观察表明，所有三个基因组中都存在对 A/T 核苷酸和 A/T 结尾密码子的偏好。此外，还证实了 11 个相同的高频密码子以及最佳表达受体。此外，还观察到 CUB 是自然选择和突变压力共同作用的结果，而自然选择是决定因素。最终，基于相对同义密码子使用（RSCU）值的相似性指数表明，亚洲三个物种的种内遗传差异较小。本研究为通过分子辅助育种提高外源基因的表达水平和优化亚洲三个物种的育种计划提供了有用的线索。
