人骨髓基质细胞的培养、扩增和分化。

Culture, Expansion and Differentiation of Human Bone Marrow Stromal Cells.

机构信息

Université de Paris, Institut de Recherche Saint-Louis, EMiLy, INSERM U1160, Paris, France.

Université de Paris, Institut de Recherche Saint-Louis, OPALE Carnot Institute, EMiLy, INSERM U1160, Paris, France.

出版信息

Methods Mol Biol. 2021;2308:3-20. doi: 10.1007/978-1-0716-1425-9_1.

DOI:10.1007/978-1-0716-1425-9_1

PMID:34057710

Abstract

Mesenchymal stromal cells (MSC) are a rare, heterogeneous and multipotent population that can be isolated from several tissues. MSC were originally discovered in the bone marrow and studied for their capacity to maintain hematopoietic cells. We will describe here methods to isolate, culture, and bank MSC from human bone marrow. Then, characterization protocols by flow cytometry, clonogenic assays and doubling time evaluation will be developed. Finally, in vitro MSC culture and differentiation into osteoblasts, adipocytes, and chondrocytes will be explained. Thus, this chapter will detail all bases to work on MSC with consensus and clear methods and protocols.

摘要

间充质基质细胞（MSC）是一种罕见的、异质性的和多能的细胞群体，可以从几种组织中分离出来。MSC 最初是在骨髓中发现的，并因其维持造血细胞的能力而被研究。在这里，我们将描述从人骨髓中分离、培养和储存 MSC 的方法。然后，将开发通过流式细胞术、克隆形成测定和倍增时间评估进行表征的方案。最后，将解释 MSC 在体外的培养和分化为成骨细胞、脂肪细胞和成软骨细胞。因此，本章将详细介绍所有基于 MSC 的工作基础，包括共识和明确的方法和方案。