基于流式细胞术的小鼠骨髓基质和血管周腔分析。
Flow Cytometry-Based Analysis of the Mouse Bone Marrow Stromal and Perivascular Compartment.
机构信息
University of Michigan School of Dentistry, Ann Arbor, MI, USA.
出版信息
Methods Mol Biol. 2021;2308:83-94. doi: 10.1007/978-1-0716-1425-9_7.
Abstract
Bone marrow stromal cells (BMSCs) account for an extremely small percentage of total bone marrow cells; therefore, it is technically challenging to harvest a good quantity of BMSCs with good viability using fluorescence-activated cell sorting (FACS). Here, we describe the methods to effectively isolate BMSCs for flow cytometry analyses and subsequent FACS. Use of transgenic reporter lines facilitates FACS-based isolation of BMSCs, aiding to uncover fundamental characteristics of these diverse cell populations.
摘要
骨髓基质细胞（BMSCs）在骨髓细胞中所占比例极小；因此，使用荧光激活细胞分选（FACS）技术从骨髓中获取大量具有良好活力的 BMSCs 具有一定的技术挑战性。在这里，我们描述了用于流式细胞术分析和随后的 FACS 的有效分离 BMSCs 的方法。转基因报告系的使用有助于基于 FACS 的 BMSCs 分离，有助于揭示这些不同细胞群体的基本特征。
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