骨髓浆细胞及其龛位的影像学研究。

Imaging of Bone Marrow Plasma Cells and of Their Niches.

机构信息

Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Rheumatology and Clinical Immunology, Berlin, Germany.

Deutsches Rheuma-Forschungszentrum (DRFZ) Berlin, a Leibniz Institute, Berlin, Germany.

出版信息

Methods Mol Biol. 2021;2308:163-176. doi: 10.1007/978-1-0716-1425-9_14.

DOI:10.1007/978-1-0716-1425-9_14

PMID:34057723

Abstract

Decade-long survival of plasma cells in the bone marrow has long been a puzzling matter. To understand how plasma cells are maintained and supported by survival-niches to account for lifelong antibody production demands new intravital imaging techniques that are able to follow up a single cell and their interaction with other cell types in situ. We achieved to successfully establish longitudinal imaging of the bone marrow (LIMB) that is based on an implantable endoscopic device. In this chapter, basic approaches on how to investigate plasma cell-stroma interaction and surgical implantation procedures are introduced.

摘要

浆细胞在骨髓中存活长达十年之久一直是一个令人费解的问题。为了了解浆细胞如何通过生存龛得到维持和支持，以解释终身产生抗体的需求，需要新的活体成像技术，这些技术能够对单个细胞及其与原位其他细胞类型的相互作用进行跟踪。我们成功地建立了基于可植入内窥镜设备的骨髓纵向成像（LIMB）。本章介绍了研究浆细胞-基质相互作用和手术植入程序的基本方法。