Walter-Brendel-Centre of Experimental Medicine, University Hospital, Ludwig-Maximilians-University Munich, BioMedical Centre, Planegg-Martinsried, Munich, Germany.
Centre Médical Universitaire (CMU), Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Methods Mol Biol. 2021;2308:139-150. doi: 10.1007/978-1-0716-1425-9_12.
The bone marrow is the major hematopoietic organ, consisting of distinct microenvironmental niches for the production of hematopoietic cells. Advanced visualizing methods are required to define and better understand the interactions between stromal and hematopoietic cells. In this chapter, we describe an ex vivo whole-mount imaging technique of the bone marrow, which allows for a fast, high-quality, and three-dimensional visualization of different bone marrow components. We provide a guide for conducting adoptive transfer experiments of fluorescently labeled leukocytes and visualizing their location in the bone marrow with respect to the bone marrow vasculature. This method presents a quick, easy, and inexpensive approach to image the bone marrow in three dimensions.
骨髓是主要的造血器官，由不同的微环境龛位组成，用于生成造血细胞。需要先进的可视化方法来定义和更好地理解基质细胞和造血细胞之间的相互作用。在本章中，我们描述了一种骨髓的体外全器官成像技术，该技术可快速、高质量地对不同的骨髓成分进行三维可视化。我们提供了一个指南，用于进行荧光标记白细胞的过继转移实验，并观察它们在骨髓中的位置与骨髓血管的关系。这种方法提供了一种快速、简单和经济的三维成像方法。
