State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, China.
Reprod Domest Anim. 2022 Feb;57(2):141-148. doi: 10.1111/rda.13974. Epub 2021 Jun 11.
Granulosa cells (GCs) play a crucial role in follicular development and atresia. Previous studies have showed that GCs in the form of monolayer influenced in vitro maturation (IVM) of oocytes. However, the effects of GCs in the form of conditioned medium and monolayer on IVM and development competence of buffalo oocytes remain unclear. In the present study, we examined the impacts of GC-conditioned medium (GCCM) and monolayer GC on maturation efficiency and embryo development of buffalo oocytes after parthenogenetic activation (PA). Our results showed that GCCM that was collected on day 2 and added to IVM medium at a 20% proportional level (2 days and 20%) exerted significant negative effects on IVM rate (41.6% vs. 44.5%), but significantly enhanced embryo development (oocyte cleavage, 81.3% vs. 69.3%; blastocyst formation, 36.3% vs. 29.3%) of buffalo oocytes after PA compared with the control group. Furthermore, monolayer GC significantly reduced both maturation efficiency (40.2% vs. 44.5%) and embryo development (oocyte cleavage, 60.6% vs. 69.3%; blastocyst formation, 20.6% vs. 29.3%) of buffalo oocytes after PA compared to the control group. Our study indicated that GCs in the form of GCCM (2 days and 20%) and monolayer GC had different effects on IVM and subsequent parthenogenetic development of buffalo oocytes.
颗粒细胞 (GCs) 在卵泡发育和闭锁中起着至关重要的作用。先前的研究表明，单层形式的 GCs 会影响卵母细胞的体外成熟 (IVM)。然而，GC 以条件培养基和单层形式对水牛卵母细胞 IVM 和发育能力的影响尚不清楚。在本研究中，我们研究了 GC 条件培养基 (GCCM) 和单层 GC 对水牛卵母细胞孤雌激活 (PA) 后成熟效率和胚胎发育的影响。我们的结果表明，在 IVM 培养基中添加 20%比例（2 天和 20%）的第 2 天收集的 GCCM 对 IVM 率（41.6%对 44.5%）有显著的负作用，但显著提高了胚胎发育（卵母细胞裂解，81.3%对 69.3%；囊胚形成，36.3%对 29.3%）。此外，与对照组相比，单层 GC 显著降低了 PA 后水牛卵母细胞的成熟效率（40.2%对 44.5%）和胚胎发育（卵母细胞裂解，60.6%对 69.3%；囊胚形成，20.6%对 29.3%）。我们的研究表明，GC 以 GCCM（2 天和 20%）和单层 GC 的形式对水牛卵母细胞的 IVM 和随后的孤雌发育有不同的影响。
