State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, China.
In Vitro Cell Dev Biol Anim. 2020 Oct;56(9):799-807. doi: 10.1007/s11626-020-00509-7. Epub 2020 Sep 30.
Granulosa cells (GCs) and theca cells (TCs) are the main components of follicles, and the interactions between GCs and TCs play a significant role in steroidogenesis, follicular growth, and atresia. However, the effects of GCs in the form of conditioned medium on steroidogenesis in buffalo TCs remain unclear. In the present study, the impacts of GC-conditioned medium (GCCM) on androgen synthesis in buffalo TCs were examined. The results showed that GCCM collected at 48 h promoted both the expression levels of androgen synthesis-related genes (CYP11A1, CYP17A1, 3β-HSD, and Star) and the secretion levels of testosterone in TCs. The treatment time of 48 h in GCCM improved both the expression levels of androgen synthesis-related genes (CYP11A1, CYP17A1, 3β-HSD, and Star) and the secretion levels of testosterone in TCs. Furthermore, GCCM that was collected at 48 h and applied to TCs for 48 h (48 h and 48 h) promoted the sensitivity of buffalo TCs to LH. This study indicated that GCCM (48 h and 48 h) enhanced the steroidogenic competence of TCs mainly through facilitating the responsiveness of TCs to LH in buffalo. This study provides a basis for further exploration of interactions between GCs and TCs for steroidogenesis in the ovary.
颗粒细胞（GCs）和膜细胞（TCs）是卵泡的主要组成部分，GCs 和 TCs 之间的相互作用在类固醇生成、卵泡生长和闭锁中起着重要作用。然而，GCs 以条件培养基的形式对水牛 TCs 类固醇生成的影响尚不清楚。本研究探讨了 GC 条件培养基（GCCM）对水牛 TCs 雄激素合成的影响。结果表明，收集的 48 h GCCM 促进了雄激素合成相关基因（CYP11A1、CYP17A1、3β-HSD 和 Star）的表达水平和 TCs 中睾酮的分泌水平。GCCM 的处理时间为 48 h 可提高雄激素合成相关基因（CYP11A1、CYP17A1、3β-HSD 和 Star）的表达水平和 TCs 中睾酮的分泌水平。此外，收集 48 h 的 GCCM 并在 48 h 时应用于 TCs（48 h 和 48 h）提高了水牛 TCs 对 LH 的敏感性。本研究表明，GCCM（48 h 和 48 h）主要通过促进 TCs 对 LH 的反应性来增强 TCs 的类固醇生成能力。本研究为进一步探索 GC 和 TCs 之间在卵巢类固醇生成中的相互作用提供了依据。
