Agudelo-Chaparro Jacqueline, Ciro-Velásquez Héctor J, Sepúlveda-Valencia José U, Pérez-Monterroza Ezequiel José
Department of Agricultural and Food Engineering, Universidad Nacional de Colombia, Sede Medellín, Medellín, Colombia.
Food Sci Technol Int. 2022 Sep;28(6):476-488. doi: 10.1177/10820132211020621. Epub 2021 May 31.
This study aimed to microencapsulate () ATCC 7469 with whey protein concentrate (WPC), maltodextrin and trehalose by spray drying and to assess the impact of microencapsulation on cell viability and the properties of the dried powders. Spray-drying conditions, including inlet air temperature, air flow rate and feed pump, were fixed as independent variables, while probiotic survival, moisture content, water activity and effective yield were established as dependent variables. The survival of encapsulated by spray drying was optimized with response surface methodology, and the stability of the powder was assessed. The optimum spray-drying conditions were an inlet air temperature, air flow rate and feed pump rate of 169 °C, 33 m/h and 16 mL/h, respectively, survival of 70%, air aspiration of 84% and outlet air temperature of 52 °C, achieving an overall desirability of 0.96. The physicochemical and structural characteristics of the produced powder were acceptable for application with regard to residual water content, hygroscopicity, water activity, and particle size. The results indicated that a protein-trehalose-maltodextrin mixture is a good wall material to encapsulate , showing important thermal protection during the drying process and increasing survival. However, a decrease in this capacity is observed at an air outlet temperature of approximately 101 °C. The possible effects of the wall materials and the drying conditions on survival are also discussed.
本研究旨在通过喷雾干燥法用乳清蛋白浓缩物（WPC）、麦芽糊精和海藻糖对嗜酸乳杆菌ATCC 7469进行微囊化，并评估微囊化对细胞活力和干粉性质的影响。将喷雾干燥条件（包括进风温度、空气流速和进料泵）设定为自变量，将益生菌存活率、水分含量、水分活度和有效产率设定为因变量。采用响应面法优化喷雾干燥法包囊嗜酸乳杆菌的存活率，并评估粉末的稳定性。最佳喷雾干燥条件为进风温度169 °C、空气流速33 m/h和进料泵速率16 mL/h，存活率为70%，抽气率为84%，出风温度为52 °C，综合可取性为0.96。就残留水分含量、吸湿性、水分活度和粒径而言，所制备粉末的物理化学和结构特性适合应用。结果表明，蛋白质-海藻糖-麦芽糊精混合物是包囊嗜酸乳杆菌的良好壁材，在干燥过程中显示出重要的热保护作用并提高了存活率。然而，在出风温度约为101 °C时，观察到这种能力有所下降。还讨论了壁材和干燥条件对存活率的可能影响。
