线粒体钙摄取评估。

Assessment of Mitochondrial Ca Uptake.

机构信息

Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Molecular Biology and Biochemistry, Medical University of Graz, Graz, Austria.

出版信息

Methods Mol Biol. 2021;2276:173-191. doi: 10.1007/978-1-0716-1266-8_13.

DOI:10.1007/978-1-0716-1266-8_13

PMID:34060041

Abstract

Mitochondrial Ca uptake regulates mitochondrial function and contributes to cell signaling. Accordingly, quantifying mitochondrial Ca signals and elaborating the mechanisms that accomplish mitochondrial Ca uptake are essential to gain our understanding of cell biology. Here, we describe the benefits and drawbacks of various established old and new techniques to assess dynamic changes of mitochondrial Ca concentration ([Ca]) in a wide range of applications.

摘要

线粒体钙摄取调节线粒体功能并有助于细胞信号转导。因此，定量测量线粒体钙信号并详细阐述完成线粒体钙摄取的机制对于深入了解细胞生物学至关重要。在这里，我们描述了各种已建立的旧技术和新技术在广泛应用中评估线粒体钙浓度([Ca])动态变化的优缺点。