Department of Cell and Developmental Biology and Consortium for Mitochondrial Research, UCL, London, UK.
Methods Mol Biol. 2022;2497:313-318. doi: 10.1007/978-1-0716-2309-1_21.
This section aims to describe the measurement of NADH and FAD levels in intact cells using fluorescence microscopy. Both NADH and FADH are major electron donors for the electron transport chain through shifting of their redox status. Furthermore, within their redox couples, only NADH and FAD are fluorescent. Therefore, calibration of the NADH and FAD fluorescence signal is a crucial factor in accurately assessing mitochondrial function and redox status.
本节旨在描述使用荧光显微镜测量完整细胞中的 NADH 和 FAD 水平。NADH 和 FADH 都是电子传递链的主要电子供体，通过它们的氧化还原状态变化来实现。此外，在它们的氧化还原对中，只有 NADH 和 FAD 是荧光的。因此，NADH 和 FAD 荧光信号的校准是准确评估线粒体功能和氧化还原状态的关键因素。
