Marine Industry Research institute for East Sea Rim (MIRE), Uljin, 36315, Republic of Korea.
Department of Life Sciences, Yeungnam University, Gyeongsan, 38541, Republic of Korea.
J Microbiol. 2021 Jul;59(7):675-680. doi: 10.1007/s12275-021-1023-x. Epub 2021 Jun 1.
Sphingorhabdus sp. YGSMI21, a novel microbial strain with an enantioselective epoxide hydrolase activity, was isolated from tidal samples contaminated by accidental oil spills subjected to enriched culture with polycyclic aromatic hydrocarbon. This strain was able to optically decompose (R)-styrene oxide (SO) and showed 100% optical purity. In addition, it showed a good enantioselectivity for the derivatives of (S)-SO, (S)-2-chlorostyrene oxide (CSO), (S)-3-CSO and (S)-4-CSO. For (S)-2-CSO, (S)-3-CSO and (S)-4-CSO, 99.9%ee was obtained with the yield of 26.2%, 24.8%, and 11.0%, respectively, when using 10 mg cells of Sphingorhabdus sp. YGSMI21 at pH 8.0 with 4 mM racemic substrates at pH 8.0 and 25°C. The values obtained in this study for (S)-2-CSO, particularly the yield of 26.2%, is noteworthy, considering that obtaining an enantiomerically pure form is difficult. Taken together, Sphingorhabdus sp. YGSMI21 can be regarded as a whole-cell biocatalyst in the production of various (S)-CSO with the chlorine group at a different position.
从受溢油污染的潮汐样品中分离到一株新型微生物菌株 Sphingorhabdus sp. YGSMI21，该菌株具有对映选择性环氧化物水解酶活性，它是通过多环芳烃富集培养得到的。该菌株能够对映选择性地分解（R）-氧化苯乙烯（SO），并显示出 100%的光学纯度。此外，它对（S）-SO、（S）-2-氯氧化苯乙烯（CSO）、（S）-3-CSO 和（S）-4-CSO 的衍生物表现出良好的对映选择性。对于（S）-2-CSO、（S）-3-CSO 和（S）-4-CSO，当使用 Sphingorhabdus sp. YGSMI21 的 10mg 细胞在 pH8.0 下，以 4mM 外消旋底物在 pH8.0 和 25°C 下反应时，分别获得了 99.9%ee 和 26.2%、24.8%和 11.0%的产率。考虑到获得对映体纯的形式很困难，本研究中获得的（S）-2-CSO 的产率（特别是 26.2%）是值得注意的。总之，Sphingorhabdus sp. YGSMI21 可以被视为一种全细胞生物催化剂，可用于生产具有不同位置氯原子的各种（S）-CSO。
