群体感应调节子 OpaR 是副溶血弧菌极性鞭毛基因的抑制剂。
The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus.
机构信息
Department of Clinical Laboratory, Nantong Third Hospital Affiliated to Nantong University, Nantong, 212006, P. R. China.
School of Medicine, Jiangsu University, Zhenjiang, 212013, P. R. China.
出版信息
J Microbiol. 2021 Jul;59(7):651-657. doi: 10.1007/s12275-021-0629-3. Epub 2021 Jun 1.
Vibrio parahaemolyticus possesses two types of flagella: a single polar flagellum (Pof) for swimming and the peritrichous lateral flagella (Laf) for swarming. Expression of Laf genes has previously been reported to be regulated by the quorum sensing (QS) regulators AphA and OpaR. In the present study, we showed that OpaR, the QS regulator at high cell density (HCD), acted as a negative regulator of swimming motility and the transcription of Pof genes in V. parahaemolyticus. OpaR bound to the promoter-proximal DNA regions of flgAMN, flgMN, and flgBCDEFGHIJ within the Pof gene loci to repress their transcription, whereas it negatively regulates the transcription of flgKL-flaC in an indirect manner. Thus, this work investigated how QS regulated the swimming motility via direct action of its master regulator OpaR on the transcription of Pof genes in V. parahaemolyticus.
副溶血性弧菌具有两种类型的鞭毛
一种用于游泳的单极鞭毛（Pof）和用于群集的周生侧鞭毛（Laf）。先前的研究报道表明，Laf 基因的表达受群体感应（QS）调节剂 AphA 和 OpaR 的调节。在本研究中，我们表明，QS 调节剂 OpaR 在高细胞密度（HCD）下作为负调控因子，调节副溶血性弧菌的游泳运动和 Pof 基因的转录。OpaR 结合到 Pof 基因座内 flgAMN、flgMN 和 flgBCDEFGHIJ 的启动子近端 DNA 区域，抑制它们的转录，而它以间接的方式负调控 flgKL-flaC 的转录。因此，这项工作研究了 QS 如何通过其主调节剂 OpaR 对副溶血性弧菌 Pof 基因转录的直接作用来调节游泳运动。
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