Che Jinyuan, Fang Qitong, Hu Shaojie, Liu Binghong, Wang Lei, Fang Xiu, Li Lekang, Luo Tuyan, Bao Baolong
Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
Biology (Basel). 2024 Jun 28;13(7):485. doi: 10.3390/biology13070485.
Porins are crucial proteins located in the outer membrane that directly influence antimicrobial resistance mechanisms and virulence in bacteria. In this study, a porin gene () was cloned in , and the function of Vp-Porin in biological characteristics and virulence was investigated. The results of sequence analysis showed that Vp-Porin is highly conserved in spp., and the predicted 3D structure showed it could form a 20-strand transmembrane β-barrel domian. Membrane permeabilization provides evidence that the membrane integrity of ∆ was damaged and the sensitivity to tetracycline, polymyxin B, rifampicin and cephalothin of ∆ obviously increased. In addition, loss of damaged motility due to downregulated flagellar synthesis. In addition, ∆ exhibited attenuated cytotoxicity to Tetrahymena. The relative survival rate of Tetrahymena infection with was 86%, which is much higher than that with WT (49%). Taken together, the results of this study indicate that Vp-Porin in plays various roles in biological characteristics in membrane integrity, antimicrobial resistance and motility and contributes to virulence.
孔蛋白是位于细菌外膜的关键蛋白质，直接影响细菌的抗菌耐药机制和毒力。在本研究中，一个孔蛋白基因（）在中被克隆，并研究了Vp - 孔蛋白在生物学特性和毒力方面的功能。序列分析结果表明，Vp - 孔蛋白在种中高度保守，预测的三维结构显示它可以形成一个20股跨膜β - 桶结构域。膜通透性实验证明，缺失株的膜完整性受损，其对四环素、多粘菌素B、利福平和头孢噻吩的敏感性明显增加。此外，缺失导致鞭毛合成下调，从而损害了运动性。此外，缺失株对四膜虫的细胞毒性减弱。感染缺失株的四膜虫相对存活率为86%，远高于感染野生型（49%）的四膜虫。综上所述，本研究结果表明，中的Vp - 孔蛋白在膜完整性、抗菌耐药性和运动性等生物学特性中发挥多种作用，并有助于毒力的产生。
