The Impact of Vp-Porin, an Outer Membrane Protein, on the Biological Characteristics and Virulence of Vibrio Parahaemolyticus.

Porins are crucial proteins located in the outer membrane that directly influence antimicrobial resistance mechanisms and virulence in bacteria. In this study, a porin gene () was cloned in , and the function of Vp-Porin in biological characteristics and virulence was investigated. The results of sequence analysis showed that Vp-Porin is highly conserved in spp., and the predicted 3D structure showed it could form a 20-strand transmembrane β-barrel domian. Membrane permeabilization provides evidence that the membrane integrity of ∆ was damaged and the sensitivity to tetracycline, polymyxin B, rifampicin and cephalothin of ∆ obviously increased. In addition, loss of damaged motility due to downregulated flagellar synthesis. In addition, ∆ exhibited attenuated cytotoxicity to Tetrahymena. The relative survival rate of Tetrahymena infection with was 86%, which is much higher than that with WT (49%). Taken together, the results of this study indicate that Vp-Porin in plays various roles in biological characteristics in membrane integrity, antimicrobial resistance and motility and contributes to virulence.