Department of Infectious Diseases, Renmin Hospital of Wuhan University, Wuhan, China.
J Med Virol. 2021 Nov;93(11):6229-6235. doi: 10.1002/jmv.27114. Epub 2021 Jun 6.
The aim of this study is to explore the relationship between liver pathological inflammation degree and pyroptosis in patients with chronic hepatitis B (CHB). One hundred and twenty CHB patients' liver tissue samples, including A0-A3 inflammatory grades, were selected. Six tissue sections were selected for each indicator in each inflammation grade. The results of immunohistochemical analysis on the pyroptosis-related molecules (NLRP3, GSDMD, caspase1, interleukin [IL]-1β, and IL-18) were determined. The correlation between the pyroptosis-related molecules and liver inflammatory activities was analyzed. The expression of NLRP3, GSDMD, caspase1, IL-18, and IL-1β was respectively significantly positively correlated with the grade of inflammatory activity (r = 0.690, p < 0.01; r = 0.681, p < 0.01; r = 0.540, p < 0.01; r = 0.725, p < 0.01; r = 0.663, p < 0.01) and linear relationship (χ = 56.763, p < 0.01; χ = 55.350, p < 0.01; χ = 34.776, p < 0.01; χ = 62.523, p < 0.01; χ = 52.521, p < 0.01) in liver tissue. The high expression of NLRP3, GSDMD, caspase1, IL-1β, and IL-18 may be involved in the process of liver tissue inflammation and damage, which is positively correlated with liver tissue inflammation in patients with CHB.
本研究旨在探讨慢性乙型肝炎（CHB）患者肝组织病理炎症程度与细胞焦亡的关系。选取 120 例 CHB 患者肝组织标本，按炎症分级 A0-A3 分为 6 组，每组每组 6 个指标，采用免疫组化法检测焦亡相关分子（NLRP3、GSDMD、caspase1、白细胞介素[IL]-1β、IL-18）的表达，并分析焦亡相关分子与肝组织炎症活动度的相关性。结果发现，NLRP3、GSDMD、caspase1、IL-18、IL-1β 的表达与炎症活动度分级呈正相关（r = 0.690，p < 0.01；r = 0.681，p < 0.01；r = 0.540，p < 0.01；r = 0.725，p < 0.01；r = 0.663，p < 0.01），且呈线性关系（χ = 56.763，p < 0.01；χ = 55.350，p < 0.01；χ = 34.776，p < 0.01；χ = 62.523，p < 0.01；χ = 52.521，p < 0.01）。肝组织中 NLRP3、GSDMD、caspase1、IL-1β、IL-18 高表达可能参与了 CHB 患者肝组织炎症损伤过程，与肝组织炎症呈正相关。
