Chen Guo-Ping, Xiang Kun, Sun Liang, Shi Yong-Lin, Meng Can, Song Lv, Liu Rui-Shan, Li Wei-Dong, Pan Hai-Feng
Department of Infectious Diseases, Anhui Center for Disease Control and Prevention, Hefei, Anhui, China.
Department of Epidemiology and Biostatistics, Anhui Medical University, Hefei, Anhui, China.
J Med Virol. 2021 Nov;93(11):6172-6179. doi: 10.1002/jmv.27115. Epub 2021 Jun 10.
Hand, foot, and mouth disease (HFMD) caused by enterovirus A71 (EV-A71) is a contagious viral disease, and toll-like receptors (TLRs) play essential roles in resisting the pathogen. The aim of this study was to assess the potential relationship between several TLRs polymorphisms and the HFMD severity in a Chinese children population. A total of 328 Chinese children with HFMD were included in the present study. The polymorphisms of TLR3 (rs3775290, rs3775291, rs3775296, rs1879026, rs5743312, rs5743313, rs5743303, rs13126816, and rs3775292), TLR4 (rs4986790, rs4986791, rs2149356, rs11536889, and rs41426344), TLR7 (rs179009, rs179010, rs179016, rs3853839, rs2302267, rs1634323, and rs5741880), and TLR8 (rs3764880, rs2159377, rs2407992, rs5744080, rs3747414, rs3764879, and rs5744069) genes were selected. The study indicated that individuals with the GG genotype of TLR3 single-nucleotide polymorphism rs1879026 had a higher risk of developing severe cases (GG vs. GT: OR = 1.875; 95% CI, 1.183-2.971; p = .007). Meanwhile, TLR3 rs3775290 CC genotype and C allele were associated with lower disease severity in females (CC vs. CT: OR = 0.350; 95% CI, 0.163-0.751; p = .006; C vs. T: OR = 0.566; 95% CI, 0.332-0.965; p = .036). TLR3 rs3775291 CC genotype showed 2.537 folds higher risk of developing severe cases in females (CC vs. CT: OR = 2.537; 95% CI, 1.108-5.806; p = .026). Moreover, TLR3 rs1879026 GG genotype was found to be related to increased risk of severe cases in males (GG vs. GT: OR = 2.076; 95% CI, 1.144-3.768; p = .016). The current findings show that the genetic variants of TLR3 rs1879026, rs3775290, and rs3775291 are associated with the severity of EV-A71-associated HFMD in a Chinese children population.
由肠道病毒A71（EV-A71）引起的手足口病（HFMD）是一种传染性病毒性疾病，而Toll样受体（TLR）在抵抗病原体方面发挥着重要作用。本研究的目的是评估中国儿童群体中几种TLR基因多态性与手足口病严重程度之间的潜在关系。本研究共纳入328名中国手足口病患儿。选取了TLR3（rs3775290、rs3775291、rs3775296、rs1879026、rs5743312、rs5743313、rs5743303、rs13126816和rs3775292）、TLR4（rs4986790、rs4986791、rs2149356、rs11536889和rs41426344）、TLR7（rs179009、rs179010、rs179016、rs3853839、rs2302267、rs1634323和rs5741880）和TLR8（rs3764880、rs2159377、rs2407992、rs5744080、rs3747414、rs3764879和rs5744069）基因的多态性。研究表明，TLR3单核苷酸多态性rs1879026的GG基因型个体发生重症病例的风险较高（GG与GT相比：OR = 1.875；95%CI，1.183 - 2.971；p = 0.007）。同时，TLR3 rs3775290的CC基因型和C等位基因与女性疾病严重程度较低相关（CC与CT相比：OR = 0.350；95%CI，0.163 - 0.751；p = 0.006；C与T相比：OR = 0.566；95%CI，0.332 - 0.965；p = 0.036）。TLR3 rs3775291的CC基因型在女性中发生重症病例的风险高2.537倍（CC与CT相比：OR = 2.537；95%CI，1.10
