Joint program of Nanchang University and Queen Mary University of London, Nanchang University, 461 Bayi Avenue, Nanchang, Jiangxi, China.
Joint program of Nanchang University and Queen Mary University of London, Nanchang University, 1299 Xuefu Avenue, Nanchang, Jiangxi, China.
Medicine (Baltimore). 2022 Jul 8;101(27):e29758. doi: 10.1097/MD.0000000000029758.
Several case-control studies have been conducted on the relationship between rs3775290 C/T and rs3853839 C/G single nucleotide polymorphisms of the Toll-like receptor (TLR) gene and hand, foot, and mouth disease (HFMD) susceptibility and severity. This meta-analysis aimed to offer a systemic review of HFMD susceptibility and severity among the Chinese Han population associated with the C/T (rs3775290) polymorphism of the TLR3 gene or C/G (rs3853839) polymorphism of the TLR7 gene.
A computer search was conducted using PubMed, Web of Science, Embase, CNKI, CBM, VIP, and WanFang databases. The time ranges were from database establishment to 30/7/2021. Articles selected according to the inclusion and exclusion criteria underwent data extraction and methodological quality evaluation. RevMan 5.4 and Stata 16.0 were adopted for meta-analysis, and the incorporated odds ratio (OR) values and 95% confidence intervals (CIs) were calculated. Sensitivity and publication bias assessments were performed.
8 articles with 9 studies were selected. Among them, there were 858 cases and 577 controls in TLR3 rs3775290 studies as well as 2151 cases and 1554 controls in TLR7 rs3853839 studies. Regarding rs3775290 of TLR3, susceptibilities of the severe type of T-possessing individuals were larger than those of C-possessing individuals [OR = 1.34, 95%CI (1.10, 1.64), P = .004]. The susceptibility of individuals with the severe TT genotype was 1.61 times that of individuals with the CC genotype [95%CI (1.07, 2.43), P=0.02], while susceptibility to HFMD was not influenced by the genotype. In terms of the rs3853839 of the TLR7 gene, C allele carriers have a higher risk of developing HFMD than G allele carriers. The susceptibility to HFMD in CC+CG individuals was 1.24 times than that in GG individuals [95%CI (1.07, 1.43), P = .004]. However, no relationship was found between this polymorphism and severity of the severe type. No significant publication bias was observed in this study.
rs3775290 (C/T) of TLR3 is associated with susceptibility to the severe type, whereas rs3853839 (C/G) of TLR7 is associated with susceptibility to HFMD. However, owing to the limited quantity and quality of the research, the aforementioned conclusions are yet to be justified by more high-quality research.
已有多项病例对照研究探讨了 Toll 样受体（TLR）基因 rs3775290 C/T 和 rs3853839 C/G 单核苷酸多态性与手足口病（HFMD）易感性和严重程度之间的关系。本荟萃分析旨在系统评价 TLR3 基因 rs3775290 C/T 多态性或 TLR7 基因 rs3853839 C/G 多态性与中国汉族人群 HFMD 易感性和严重程度的关系。
计算机检索 PubMed、Web of Science、Embase、中国知网（CNKI）、中国生物医学文献数据库（CBM）、维普数据库（VIP）和万方数据库，检索时间均从数据库建立至 2021 年 7 月 30 日。根据纳入和排除标准筛选文献后进行数据提取和方法学质量评价，采用 RevMan 5.4 和 Stata 16.0 软件进行荟萃分析，计算合并的比值比（OR）值及其 95%置信区间（CI）。进行敏感性分析和发表偏倚评估。
共纳入 8 篇文献 9 项研究，其中 TLR3 rs3775290 研究纳入 858 例病例和 577 例对照，TLR7 rs3853839 研究纳入 2151 例病例和 1554 例对照。对于 TLR3 的 rs3775290，携带 T 等位基因的个体患重型的易感性大于携带 C 等位基因的个体[OR=1.34，95%CI（1.10，1.64），P=0.004]。携带 TT 基因型的个体患重型的易感性是 CC 基因型个体的 1.61 倍[95%CI（1.07，2.43），P=0.02]，而 HFMD 的易感性不受基因型影响。对于 TLR7 的 rs3853839，携带 C 等位基因的个体患 HFMD 的风险高于携带 G 等位基因的个体。CC+CG 个体患 HFMD 的易感性是 GG 个体的 1.24 倍[95%CI（1.07，1.43），P=0.004]。但是，该多态性与重型的严重程度之间无关联。本研究未发现明显的发表偏倚。
TLR3 的 rs3775290（C/T）与重型易感性相关，而 TLR7 的 rs3853839（C/G）与 HFMD 易感性相关。但是，由于研究数量和质量有限，上述结论还需要更多高质量研究来验证。
