College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China.
Department of Blood Transfusion, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710069, China.
Anal Biochem. 2021 Aug 15;627:114265. doi: 10.1016/j.ab.2021.114265. Epub 2021 May 29.
Point-of-care testing (POCT) demands for rapidly obtaining test results by means of portable analytical instruments and auxiliary reagents at the sampling site. It's important for tumor marker to be recognized and detected in early clinical diagnosis. Many studies focused on producing small portable devices that would allow fast, accurate, and on-site detection. This study aimed to report a magnetic quantitative lateral flow immunoassay (LFIA) system based on poly (acrylic acid) (PAA)-modified gold magnetic nanoparticles (PGMNs) for detecting prostate-specific antigen (PSA) qualitatively and quantitatively. The result was easily achievable with a portable magnetic reader within 15 min. Under optimal conditions, as low as 0.17 ng/mL PSA could be detected. The method was validated using a well-established Solin electrochemiluminescence immunoassay and showed high consistency in detecting 84 serum samples (R = 0.98). The quantitative LFIA based on PGMNs established in this study was proven to be rapid, accurate, sensitive, and inexpensive. As a POCT, it can be potentially developed for the quantitative diagnosis of other disease-related protein biomarkers.
即时检测（POCT）要求通过便携式分析仪器和辅助试剂在采样现场快速获得检测结果。肿瘤标志物的识别和检测在早期临床诊断中很重要。许多研究都集中在生产小型便携式设备上，以实现快速、准确和现场检测。本研究旨在报告一种基于聚丙烯酸（PAA）修饰的金磁纳米颗粒（PGMNs）的磁性定量侧向流动免疫分析（LFIA）系统，用于定性和定量检测前列腺特异性抗原（PSA）。在 15 分钟内，使用便携式磁阅读器即可轻松获得检测结果。在最佳条件下，可检测到低至 0.17ng/mL 的 PSA。该方法使用成熟的 Solin 电化学发光免疫分析法进行验证，在检测 84 份血清样本时显示出高度一致性（R=0.98）。本研究建立的基于 PGMNs 的定量 LFIA 被证明具有快速、准确、灵敏和经济的特点。作为一种 POCT，它可以潜在地开发用于定量诊断其他与疾病相关的蛋白质生物标志物。
