肠道沙门氏菌亚种肠道病原体相互作用及其在胆囊癌中的意义。

Salmonella enterica subsp. enterica host-pathogen interactions and their implications in gallbladder cancer.

机构信息

Indian Council of Medical Research-National AIDS Research Institute, Pune, Maharashtra, 411026, India.

Department of Molecular and Human Genetics, Jiwaji University, Gwalior, MP, 474001, India.

出版信息

Microb Pathog. 2021 Aug;157:105011. doi: 10.1016/j.micpath.2021.105011. Epub 2021 May 29.

DOI:10.1016/j.micpath.2021.105011

PMID:34062227

Abstract

BACKGROUND

Several studies have linked chronic typhoid infection with gallbladder carcinoma without completely understood mechanism. This study was performed in order to understand role of Salmonella in gallbladder cancer etiology.

METHODS

Known Salmonella host-pathogen interactions were screened from database in addition to known gallbladder carcinoma targets. Host-pathogen interaction map of S. enterica was prepared and screened for interactions with gallbladder carcinoma targets. Further functional overrepresentation analysis was performed to understand the role of human targets involved in Salmonella host-pathogen interactions in gallbladder carcinoma.

RESULTS

Salmonella interact with several human proteins involved in gallbladder carcinoma. MAPK and RAC1 are the most important human proteins based on node degree value among all GBC associated interactors identified in current data search. Functional over-representation analysis reveals that Salmonella can induce adenocarcinoma which constitutes 85% of gallbladder cancer.

CONCLUSION

Though, the role of MAPK/ERK and PI3K/AKT/mTOR pathway is already suggested for Salmonella mediated gallbladder cancer, but current data based approach indicate several new insight for exploration of the role of Salmonella in gallbladder cancer etiology. The results indicate about several other processes including CREB/SP-1 and BSG(CD147) signaling, that must be given consideration for understanding the role of Salmonella in gallbladder cancer.

摘要

背景

几项研究表明，慢性伤寒感染与胆囊癌有关，但具体机制尚不完全清楚。本研究旨在探讨沙门氏菌在胆囊癌发病机制中的作用。

方法

从数据库中筛选已知的沙门氏菌宿主-病原体相互作用，以及已知的胆囊癌靶标。制备并筛选沙门氏菌肠亚种的宿主-病原体相互作用图谱，以研究与胆囊癌靶标相互作用的人类靶点。进一步进行功能过表达分析，以了解参与沙门氏菌宿主-病原体相互作用的人类靶点在胆囊癌中的作用。

结果

沙门氏菌与几种参与胆囊癌的人类蛋白相互作用。在当前数据搜索中确定的所有与 GBC 相关的相互作用体中，根据节点度值，MAPK 和 RAC1 是最重要的人类蛋白。功能过表达分析表明，沙门氏菌可诱导构成胆囊癌 85%的腺癌。

结论

虽然 MAPK/ERK 和 PI3K/AKT/mTOR 途径的作用已被提出用于沙门氏菌介导的胆囊癌，但基于当前数据的方法为探索沙门氏菌在胆囊癌发病机制中的作用提供了一些新的见解。研究结果表明，沙门氏菌可能通过其他几个过程，包括 CREB/SP-1 和 BSG(CD147)信号通路，来发挥作用，这对于理解沙门氏菌在胆囊癌中的作用必须加以考虑。