Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, 410128, China.
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, 410128, China; Hunan Co-Innovation Center of Animal Production Safety, Changsha, Hunan Province, 410128, China.
Vet Parasitol. 2021 Jul;295:109475. doi: 10.1016/j.vetpar.2021.109475. Epub 2021 May 27.
Swine stephanuriasis caused by kidney worm Stephanurus dentatus is a parasitic disease in tropical and subtropical countries, leading to economic losses. Despite its significance as a pathogen, the phylogenetic position and taxonomic status of this nematode remain poorly understood. Mitochondrial (mt) genome sequences are known to provide useful genetic markers for investigations in these areas, but mt genome sequences are lacking for S. dentatus. In the present study, we determined the complete mt genome sequences of S. dentatus with an Illumina platform and compared it with the mt genomes of other closely related species. The circular mt genome was 13,735 bp in size with 36 genes. All genes are transcribed in the same direction and the mt gene arrangement is identified as a GA3 pattern, that is the most common pattern of gene arrangement observed in nematodes to date. Phylogenetic analysis using concatenated amino acid sequences of 12 protein-coding genes supported the hypothesis that S. dentatus was closely related to the family Chabertiidae. Our results provided insights into the phylogenetic relationship of the family Syngamidae within the superfamily Strongyloidea.
由肾膨结线虫 Stephanurus dentatus 引起的猪旋毛虫病是热带和亚热带国家的一种寄生虫病，导致经济损失。尽管它是一种重要的病原体，但这种线虫的系统发育位置和分类地位仍知之甚少。线粒体（mt）基因组序列被认为是这些领域研究的有用遗传标记，但 S. dentatus 的 mt 基因组序列尚不清楚。在本研究中，我们使用 Illumina 平台确定了 S. dentatus 的完整 mt 基因组序列，并将其与其他密切相关物种的 mt 基因组进行了比较。圆形 mt 基因组大小为 13735bp，包含 36 个基因。所有基因都以相同的方向转录，mt 基因排列被确定为 GA3 模式，这是迄今为止在线虫中观察到的最常见的基因排列模式。使用 12 个蛋白质编码基因的串联氨基酸序列进行的系统发育分析支持了 S. dentatus 与 Chabertiidae 科密切相关的假设。我们的研究结果为 Syngamidae 科在 Strongyloidea 超科中的系统发育关系提供了新的见解。
