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优化二嵌段类弹性蛋白多肽自组装成纳米颗粒的设计。

Refining the Design of Diblock Elastin-Like Polypeptides for Self-Assembly into Nanoparticles.

作者信息

Dai Michèle, Georgilis Evangelos, Goudounet Guillaume, Garbay Bertrand, Pille Jan, van Hest Jan C M, Schultze Xavier, Garanger Elisabeth, Lecommandoux Sébastien

机构信息

University Bordeaux, CNRS, Bordeaux INP, LCPO, UMR 5629, 33600 Pessac, France.

L'Oréal Recherche Avancée, 1 Avenue Eugène Schueller, 93600 Aulnay-sous-Bois, France.

出版信息

Polymers (Basel). 2021 May 1;13(9):1470. doi: 10.3390/polym13091470.

DOI:10.3390/polym13091470
PMID:34062852
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8125372/
Abstract

Diblock copolymers based-on elastin-like polypeptide (ELP) have the potential to undergo specific phase transitions when thermally stimulated. This ability is especially suitable to form carriers, micellar structures for instance, for delivering active cargo molecules. Here, we report the design and study of an ELP diblock library based on ELP-[MV-]-[I-]. First, ELP-[MV-]-[I-] ( = , , ; = , ) that showed a similar self-assembly propensity (unimer-to-aggregate transition) as their related monoblocks ELP-[MV-] and ELP-[I-]. By selectively oxidizing methionines of ELP-[MV-] within the different diblocks structures, we have been able to access a thermal phase transition with three distinct regimes (unimers, micelles, aggregates) characteristic of well-defined ELP diblocks.

摘要

基于类弹性蛋白多肽（ELP）的二嵌段共聚物在热刺激下有可能发生特定的相变。这种能力特别适合用于形成载体，例如胶束结构，用于递送活性货物分子。在此，我们报告了基于ELP-[MV-]-[I-]的ELP二嵌段文库的设计与研究。首先，ELP-[MV-]-[I-]（ = ， ， ； = ， ）表现出与其相关单嵌段ELP-[MV-]和ELP-[I-]相似的自组装倾向（单体到聚集体转变）。通过在不同的二嵌段结构中选择性氧化ELP-[MV-]的甲硫氨酸，我们能够实现具有三种不同状态（单体、胶束、聚集体）的热相变，这是明确的ELP二嵌段所特有的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/0ed6a5a3bbef/polymers-13-01470-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/5e390446617c/polymers-13-01470-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/249cc40eb4c8/polymers-13-01470-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/87a9677c9d2c/polymers-13-01470-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/ee660626ee15/polymers-13-01470-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/a585bd206246/polymers-13-01470-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/95d8173867c0/polymers-13-01470-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/94b1961ab5ba/polymers-13-01470-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/0ed6a5a3bbef/polymers-13-01470-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/5e390446617c/polymers-13-01470-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/249cc40eb4c8/polymers-13-01470-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/87a9677c9d2c/polymers-13-01470-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/ee660626ee15/polymers-13-01470-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/a585bd206246/polymers-13-01470-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/95d8173867c0/polymers-13-01470-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/94b1961ab5ba/polymers-13-01470-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea21/8125372/0ed6a5a3bbef/polymers-13-01470-g008.jpg

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