Rosselin Marie, Chinoy Zoeisha S, Bravo-Anaya Lourdes Mónica, Lecommandoux Sébastien, Garanger Elisabeth
Université de Bordeaux, CNRS, Bordeaux INP, LCPO, UMR 5629, Pessac F-33600, France.
ACS Macro Lett. 2021 Jan 19;10(1):65-70. doi: 10.1021/acsmacrolett.0c00775. Epub 2020 Dec 17.
A library of synthetic elastin-like glycopolypeptides were synthesized and screened by microscale thermophoresis to identify key structural parameters affecting lectin binding efficacy. While polypeptide backbone size and glycovalency were found to have little influence, the presence of a linker at the anomeric position of galactose and the absence of positive charge on the polypeptide residue holding the sugar unit were found to be critical for the binding to RCA120.
合成了一个类弹性蛋白糖多肽文库，并通过微量热泳进行筛选，以确定影响凝集素结合效率的关键结构参数。虽然发现多肽主链大小和糖价影响不大，但发现半乳糖异头位置存在连接子以及携带糖单元的多肽残基上没有正电荷对于与RCA120的结合至关重要。
