含蛋氨酸的弹性蛋白样多肽的定量侧链修饰作为调节其性质的通用工具
Quantitative Side-Chain Modifications of Methionine-Containing Elastin-Like Polypeptides as a Versatile Tool to Tune Their Properties.
作者信息
Kramer Jessica R, Petitdemange Rosine, Bataille Laure, Bathany Katell, Wirotius Anne-Laure, Garbay Bertrand, Deming Timothy J, Garanger Elisabeth, Lecommandoux Sebastien
机构信息
Department of Chemistry and Biochemistry and Department of Bioengineering, University of California, Los Angeles, California 90095, United States.
Université de Bordeaux/Bordeaux INP, ENSCBP, 16 avenue Pey-Berland, Pessac 33607, France.
出版信息
ACS Macro Lett. 2015 Nov 17;4(11):1283-1286. doi: 10.1021/acsmacrolett.5b00651. Epub 2015 Nov 3.
Tuning the lower critical solution temperature (LCST) of temperature-responsive recombinant elastin-like polypeptides has usually been achieved by designing different protein sequences, in terms of amino acid composition and length, implying tedious molecular cloning steps. In the present work, we have explored the chemoselective alkylation of methionine as an easy means to modify elastin repeat side chains and easily modulate the LCST of the polypeptides. Such a versatile synthetic method shall practically be exploited to modulate any properties of recombinant polymers.
调节温度响应性重组弹性蛋白样多肽的下临界溶液温度（LCST）通常是通过设计不同的蛋白质序列来实现的，这涉及氨基酸组成和长度，意味着需要繁琐的分子克隆步骤。在本研究中，我们探索了甲硫氨酸的化学选择性烷基化，作为一种修饰弹性蛋白重复侧链并轻松调节多肽LCST的简便方法。这种通用的合成方法实际上可用于调节重组聚合物的任何性质。
Zotero 插件