Minozzi Giulietta, Lazzari Barbara, De Iorio Maria Grazia, Costa Cecilia, Carpana Emanuele, Crepaldi Paola, Rizzi Rita, Facchini Elena, Gandini Gustavo, Stella Alessandra, Pagnacco Giulio
Department of Veterinary Medicine DIMEVET, University of Milan, 20133 Milan, Italy.
IBBA-CNR, 20133 Milano, Italy.
Animals (Basel). 2021 May 2;11(5):1311. doi: 10.3390/ani11051311.
At the end of the last glaciation, was established in northern Europe. In Italy, adapted to the mild climate and to the rich floristic biodiversity. Today, with the spread of and with the increasing use of pesticides in agriculture, the Ligustica subspecies is increasingly dependent on human action for its survival. In addition, the effects of globalization of bee keeping favored the spread in Italy of other honeybee stocks of , in particular the Buckfast bee. The purpose of this study was to characterize the Italian honeybee's population by sequencing the whole genome of 124 honeybees. Whole genome sequencing was performed by Illumina technology, obtaining a total coverage of 3720.89X, with a mean sample coverage of 29.77X. A total of 4,380,004 SNP variants, mapping on Amel_HAv3.1 chromosomes, were detected. Results of the analysis of the patterns of genetic variation allowed us to identify and subgroup bees according to their type. The investigation revealed the genetic originality of the Sicula, and in limited genetic introgression from the other breeds. Morphometric analysis of 5800 worker bees was in agreement with genomic data.
在上一个冰期末期，[物种名称]在北欧得以确立。在意大利，[物种名称]适应了温和的气候以及丰富的植物多样性。如今，随着[物种名称]的传播以及农业中杀虫剂使用的增加，利古斯蒂卡亚种在生存方面越来越依赖人类活动。此外，养蜂全球化的影响促使其他[物种名称]蜜蜂种群在意大利扩散，尤其是巴克法斯特蜜蜂。本研究的目的是通过对124只蜜蜂的全基因组进行测序来表征意大利蜜蜂种群。全基因组测序采用Illumina技术进行，总覆盖度为3720.89X，平均样本覆盖度为29.77X。共检测到4380004个单核苷酸多态性（SNP）变异，这些变异定位在Amel_HAv3.1染色体上。遗传变异模式的分析结果使我们能够根据蜜蜂的类型对其进行识别和分组。调查揭示了西西里蜜蜂的遗传独特性，并且[物种名称]中来自其他品种的遗传渗入有限。对5800只工蜂的形态测量分析与基因组数据一致。
