Indivenire srl, Via Alla Cascata 56/C, 38123 Trento, Italy.
Department of Engineering, University of Campania "L. Vanvitelli", Via Roma 29, 81031 Aversa, Italy.
Sensors (Basel). 2021 May 15;21(10):3443. doi: 10.3390/s21103443.
Postoperative pancreatic fistula (POPF), the major driver of morbidity and mortality following pancreatectomy, is caused by an abnormal communication between the pancreatic ductal epithelium and another epithelial surface containing pancreas-derived, enzyme-rich fluid. There is a strong correlation between the amylase content in surgically-placed drains early in the postoperative course and the development of POPF. A simple and cheap method to determine the amylase content from the drain effluent has been eagerly advocated. Here, we developed an amylase optical biosensor, based on a surface plasmon resonance (SPR) plastic optical fiber (POF), metallized with a 60 nm layer of gold and interrogated with white light. The sensor was made specific by coupling it with an anti-amylase antibody. Each surface derivatization step was optimized and studied by XPS, contact angle, and fluorescence. The POF-biosensor was tested for its response to amylase in diluted drain effluents. The volume of sample required was 50 µL and the measurement time was 8 min. The POF-biosensor showed selectivity for amylase, a calibration curve log-linear in the range of 0.8-25.8 U/L and a limit of detection (LOD) of ~0.5 U/L. In preliminary tests, the POF-biosensor allowed for the measurement of the amylase content of diluted surgically-placed drain effluents with an accuracy of >92% with respect to the gold standard. The POF-biosensor allows for reliable measurement and could be implemented to allow for a rapid bedside assessment of amylase value in drains following pancreatectomy.
术后胰腺瘘（POPF）是胰腺切除术后发病率和死亡率的主要原因，是由胰腺导管上皮和另一个含有胰腺来源的富含酶的液体的上皮表面之间的异常沟通引起的。在术后早期放置的引流管中的淀粉酶含量与 POPF 的发展之间存在很强的相关性。人们迫切提倡一种简单廉价的方法来确定引流液中的淀粉酶含量。在这里，我们开发了一种基于表面等离子体共振（SPR）塑料光纤（POF）的淀粉酶光学生物传感器，该传感器用 60nm 厚的金层进行金属化，并使用白光进行检测。通过与抗淀粉酶抗体偶联，使传感器具有特异性。通过 XPS、接触角和荧光研究，对每个表面衍生化步骤进行了优化和研究。测试了 POF 生物传感器对稀释引流液中淀粉酶的反应。所需的样品体积为 50µL，测量时间为 8 分钟。POF 生物传感器对淀粉酶表现出选择性，在 0.8-25.8U/L 的范围内呈对数线性校准曲线，检测限（LOD）约为 0.5U/L。在初步测试中，POF 生物传感器允许以 >92%的准确度测量稀释的手术放置引流液中的淀粉酶含量，与金标准相比。POF 生物传感器允许进行可靠的测量，并可用于快速床边评估胰腺切除术后引流管中的淀粉酶值。
