Vasilevskaya Ekaterina, Makarenko Aleksandr, Tolmacheva Galina, Chernukha Irina, Kibitkina Anastasiya, Fedulova Liliya
V.M. Gorbatov Federal Research Center for Food Systems, Russian Academy of Sciences, 109316 Moscow, Russia.
Biomedicines. 2021 May 21;9(6):585. doi: 10.3390/biomedicines9060585.
(1) Background: Hemorrhagic stroke is a lethal disease, accounting for 15% of all stroke cases. However, there are very few models of stroke with a hemorrhagic etiology. Research work is devoted to studying the development of cerebrovascular disorders in rats with an intracerebral hematoma model. The aim of this study was to conduct a comprehensive short-term study, including neurological tests, biochemical blood tests, and histomorphological studies of brain structures. (2) Methods: The model was reproduced surgically by traumatizing the brain in the capsula interna area and then injecting autologous blood. Neurological deficit was assessed according to the McGrow stroke-index scale, motor activity, orientation-exploratory behavior, emotionality, and motor functions. On Day 15, after the operation, hematological and biochemical blood tests as well as histological studies of the brain were performed. (3) Results: The overall lethality of the model was 43.7%. Acute intracerebral hematoma in rats causes marked disorders of motor activity and functional impairment, as well as inflammatory processes in the nervous tissue, which persist for at least 14 days. (4) Conclusions: This model reflects the situation observed in the clinic and reproduces the main diagnostic criteria for acute disorders of cerebral circulation.
(1) 背景：出血性中风是一种致命疾病，占所有中风病例的15%。然而，病因是出血性的中风模型非常少。研究工作致力于研究脑内血肿模型大鼠脑血管疾病的发展。本研究的目的是进行一项全面的短期研究，包括神经学测试、血液生化检测以及脑结构的组织形态学研究。(2) 方法：通过在内囊区损伤大脑然后注入自体血，以手术方式复制该模型。根据McGrow中风指数量表、运动活动、定向探索行为、情绪及运动功能评估神经功能缺损。术后第15天，进行血液学和血液生化检测以及脑组织学研究。(3) 结果：该模型的总体致死率为43.7%。大鼠急性脑内血肿会导致明显的运动活动障碍和功能损害，以及神经组织中的炎症过程，这些情况至少持续14天。(4) 结论：该模型反映了临床观察到的情况，并再现了急性脑循环障碍的主要诊断标准。
