Regenerative Bioscience Center, University of Georgia, Athens, GA, United States; Neuroscience Program, Biomedical and Health Sciences Institute, University of Georgia, Athens, GA, United States; Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA, United States.
Regenerative Bioscience Center, University of Georgia, Athens, GA, United States; Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA, United States.
Brain Res. 2020 Jun 1;1736:146778. doi: 10.1016/j.brainres.2020.146778. Epub 2020 Mar 16.
The acute stroke phase is a critical time frame used to evaluate stroke severity, therapeutic options, and prognosis while also serving as a major tool for the development of diagnostics. To further understand stroke pathophysiology and to enhance the development of treatments, our group developed a translational pig ischemic stroke model. In this study, the evolution of acute ischemic tissue damage, immune responses, and functional deficits were further characterized. Stroke was induced by middle cerebral artery occlusion in Landrace pigs. At 24 h post-stroke, magnetic resonance imaging revealed a decrease in ipsilateral diffusivity, an increase in hemispheric swelling resulting in notable midline shift, and intracerebral hemorrhage. Stroke negatively impacted white matter integrity with decreased fractional anisotropy values in the internal capsule. Like patients, pigs showed a reduction in circulating lymphocytes and a surge in neutrophils and band cells. Functional responses corresponded with structural changes through reductions in open field exploration and impairments in spatiotemporal gait parameters. Characterization of acute ischemic stroke in pigs provided important insights into tissue and functional-level assessments that could be used to identify potential biomarkers and improve preclinical testing of novel therapeutics.
急性卒中阶段是一个关键的时间框架，用于评估卒中严重程度、治疗选择和预后，同时也是诊断发展的主要工具。为了进一步了解卒中的病理生理学，并增强治疗方法的开发，我们的团队开发了一种转化猪缺血性卒中模型。在这项研究中，进一步描述了急性缺血性组织损伤、免疫反应和功能缺陷的演变。通过大脑中动脉闭塞在长白猪中诱导卒中。在卒中后 24 小时，磁共振成像显示对侧弥散度降低，半球肿胀导致明显的中线移位，以及颅内出血。卒中导致白质完整性受损，内囊的各向异性分数值降低。与患者一样，猪的循环淋巴细胞减少，中性粒细胞和带状细胞增加。功能反应与结构变化相对应，表现在旷场探索减少和时空步态参数受损。猪的急性缺血性卒中的特征提供了对组织和功能水平评估的重要见解，可用于识别潜在的生物标志物并改善新型治疗方法的临床前测试。
