波兰手工奶酪中[具体物质]的存在及其基质辅助激光解吸电离飞行时间质谱鉴定
Occurrence of in Artisanal Cheeses from Poland and Its Identification by MALDI-TOF MS.
作者信息
Pyz-Łukasik Renata, Gondek Michał, Winiarczyk Dagmara, Michalak Katarzyna, Paszkiewicz Waldemar, Piróg-Komorowska Anna, Policht Agata, Ziomek Monika
机构信息
Department of Food Hygiene of Animal Origin, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka, 12, 20-033 Lublin, Poland.
Department and Clinic of Animal Internal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Głęboka, 30, 20-612 Lublin, Poland.
出版信息
Pathogens. 2021 May 21;10(6):632. doi: 10.3390/pathogens10060632.
is a foodborne pathogen. A source of infection can be artisanal cheeses. Identification of the species is important for the protection of public health and the food industry. This study aimed to examine artisanal cheeses for the presence of and the effectiveness of the MALDI-TOF MS method in the identification of the isolates. A total of 370 samples of artisanal cheeses were examined. was found in 23 cheese samples (6.2%). The reliability of identification achieved by MALDI-TOF MS was varied, and the vast majority of the isolates (27/32) were identified only to the secure genus, probable species level. This study showed that (i) the occurrence of in the artisanal cheeses was at a higher level than that in the other EU countries, (ii) the standard of species identification of isolates from artisanal cheeses achieved by MALDI-TOF MS was not satisfactory and (iii) the presence of in artisanal cheeses remains a problem with regard to the food safety criterion and a potential public health risk.
是一种食源性病原体。感染源可能是手工制作的奶酪。该菌种的鉴定对于保护公众健康和食品工业很重要。本研究旨在检测手工制作的奶酪中是否存在该菌种，以及基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）方法在鉴定该菌种分离株方面的有效性。共检测了370份手工制作奶酪的样本。在23份奶酪样本（6.2%）中发现了该菌种。通过MALDI-TOF MS实现的该菌种鉴定的可靠性各不相同，绝大多数分离株（27/32）仅被鉴定到可靠的属、可能的种水平。本研究表明：（i）手工制作奶酪中该菌种的发生率高于其他欧盟国家；（ii）通过MALDI-TOF MS从手工制作奶酪中鉴定该菌种分离株的物种鉴定标准并不令人满意；（iii）就食品安全标准和潜在的公共健康风险而言，手工制作奶酪中该菌种的存在仍然是一个问题。
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