利用随机扩增多态性DNA标记分析三倍体和二倍体（Burchell，1822）之间的遗传变异

Genetic Variation between Triploid and Diploid (Burchell, 1822) Using RAPD Markers.

作者信息

Normala Jalil, Okomoda Victor Tosin, Mohd Azizul Alim, Nur Asma Ariffin, Abol-Munafi Ambok Bolong, Md Sheriff Shahreza

机构信息

Faculty of Food Science and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus 21030, Malaysia.

Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture, Makurdi P.M.B. 2373, Nigeria.

出版信息

Vet Sci. 2021 May 4;8(5):75. doi: 10.3390/vetsci8050075.

DOI:10.3390/vetsci8050075

PMID:34064306

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8147776/

Abstract

This study was designed to examine the use of RAPD markers in discriminating triploid and diploid African catfish (Burchell, 1822). Following a routine technique, triploidy was induced by cold shock and confirm by erythrocyte measurement in . Thereafter, 80 RAPD markers were screened; out of which, three showed the highest percentage of polymorphism (i.e., OPB 16 = 71.43%; OPC 14 = 61.9%; OPD 12 = 75%). The results obtained showed genotype differences between triploid and diploid without overlapping. However, the development of a Sequence Characterized Amplified Region (SCAR) marker was not achievable because progenies of triploid and diploid could not be differentiated based on a specific fragment. Consequently, the genetic distance showed high similarities for both treatments and the UPGMA-generated dendrogram could not separate the treatments into two distinct clusters. It was concluded that RAPD makers cannot be used to separate the ploidy status of fishes.

摘要

本研究旨在探讨随机扩增多态性DNA（RAPD）标记在鉴别三倍体和二倍体非洲鲶鱼（Burchell，1822）中的应用。采用常规技术，通过冷休克诱导三倍体，并通过红细胞测量进行确认。此后，筛选了80个RAPD标记；其中，三个显示出最高的多态性百分比（即OPB 16 = 71.43%；OPC 14 = 61.9%；OPD 12 = 75%）。所得结果表明三倍体和二倍体之间存在基因型差异且无重叠。然而，由于三倍体和二倍体的后代无法基于特定片段进行区分，因此无法开发序列特异性扩增区域（SCAR）标记。因此，两种处理的遗传距离显示出高度相似性，并且基于非加权组平均法（UPGMA）生成的树状图无法将处理分为两个不同的聚类。得出的结论是，RAPD标记不能用于区分鱼类的倍性状态。