Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture, P.M.B., 2373, Makurdi, Nigeria.
Institute of Tropical Aquaculture and Fisheries Research (AQUATROP), Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia.
Sci Rep. 2020 Feb 12;10(1):2425. doi: 10.1038/s41598-020-59389-2.
This study investigated the use of electric-shock in inducing triploidy in African catfish Clarias gariepinus. To achieve this, three voltages (9, 12, 21 V) were applied for different durations (3, 5, 10 min). The shock was initiated approximately three minutes after fertilization followed by incubation in ambient temperature. After incubation, hatchability and survival rates were determined while ploidy status of the treatment fishes was confirmed in one-month-old fingerlings using the exclusive triploid range of the erythrocyte major axis previously reported for the same species (11.9-14.9 μm) and by cytogenetic analysis of the chromosome. The results showed triploidy were achieved in 10 to 85% of the treatment groups. A consistent trend of decrease in hatchability and an increase in triploidy rate was observed with increased electroporation voltages and shock durations. The mean erythrocyte major axis length of triploid progenies (3n = 84) was observed to be between 11.3-14.6 μm and was higher than the range of 7.0-10.5 μm recorded for diploid progenies (2n = 56). It was concluded that electric shock can be used to induce triploidy in African catfish C. gariepinus.
本研究探讨了电击诱导非洲鲶（Clarias gariepinus）三倍体的方法。为此，使用了三种电压（9、12 和 21V），并施加不同的时长（3、5 和 10 分钟）。电击在受精后大约三分钟开始，然后在环境温度下孵育。孵育后，测定孵化率和存活率，同时通过血细胞主轴的独特三倍体范围（先前报道的同种鱼类为 11.9-14.9μm）和染色体的细胞遗传学分析，确认处理鱼类的倍性状态。结果表明，处理组中有 10%至 85%的鱼产生了三倍体。随着电穿孔电压和电击时长的增加，孵化率持续下降，三倍体率增加。三倍体后代（3n=84）的平均红细胞主轴长度为 11.3-14.6μm，高于二倍体后代（2n=56）的 7.0-10.5μm 范围。结论是，电击可以用于诱导非洲鲶 C. gariepinus 的三倍体。
