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全子孢子疫苗诱导的保护性免疫引发特异性B细胞抗原。

Protective Immunity Induced by an Whole Sporozoite Vaccine Elicits Specific B-Cell Antigens.

作者信息

Juárez-Estrada Marco A, Gayosso-Vázquez Amanda, Tellez-Isaias Guillermo, Alonso-Morales Rogelio A

机构信息

Departamento de Medicina y Zootecnia de Aves, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, Cd. De México 04510, Mexico.

Departamento de Genética y Bioestadística, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, Cd. De México 04510, Mexico.

出版信息

Animals (Basel). 2021 May 9;11(5):1344. doi: 10.3390/ani11051344.

DOI:10.3390/ani11051344
PMID:34065041
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8151427/
Abstract

This study investigated protection against following the vaccination of chicks with 5.3 × 10 whole-sporozoites emulsified in the nanoparticle adjuvant IMS 1313 N VG Montanide™ (EtSz-IMS1313). One-day-old specific pathogen-free (SPF) chicks were subcutaneously injected in the neck with EtSz-IMS1313 on the 1st and 10th days of age. Acquired immunity was assayed through a challenge with 3 × 10 homologous sporulated oocysts at 21 days of age. The anticoccidial index (ACI) calculated for every group showed the effectiveness of EtSz-IMS1313 as a vaccine with an ACI of 186; the mock-injected control showed an ACI of 18 and the unimmunized, challenged control showed an ACI of -28. In a comparison assay, antibodies from rabbits and SPF birds immunized with EtSz-IMS1313 recognized almost the same polypeptides in the blotting of sporozoites and merozoites. However, rabbit antisera showed the clearest recognition pattern. Polypeptides of 120, 105, 94, 70, 38, and 19 kDa from both life cycle stages were the most strongly recognized by both animal species. The zoite-specific IgG antibodies from the rabbits demonstrated the feasibility for successful B cell antigen identification.

摘要

本研究调查了用纳米颗粒佐剂IMS 1313 N VG Montanide™（EtSz-IMS1313）乳化的5.3×10个全子孢子对雏鸡进行疫苗接种后的保护作用。1日龄无特定病原体（SPF）雏鸡在1日龄和10日龄时于颈部皮下注射EtSz-IMS1313。在21日龄时用3×10个同源有孢子卵囊进行攻毒来测定获得性免疫力。为每组计算的抗球虫指数（ACI）显示EtSz-IMS1313作为疫苗的有效性，ACI为186； mock注射对照组的ACI为18，未免疫攻毒对照组的ACI为 -28。在一项比较试验中，用EtSz-IMS1313免疫的兔和SPF鸡的抗体在子孢子和裂殖子的印迹中识别几乎相同的多肽。然而，兔抗血清显示出最清晰的识别模式。来自两个生命周期阶段的120、105、94、70、38和19 kDa的多肽被两种动物最强烈地识别。兔的动合子特异性IgG抗体证明了成功鉴定B细胞抗原的可行性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/8151427/6fa8fc69b381/animals-11-01344-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/8151427/386c6b323baf/animals-11-01344-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/8151427/b4bf07e5bd24/animals-11-01344-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/8151427/700dc87b36ff/animals-11-01344-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/8151427/24624740bbcf/animals-11-01344-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/8151427/6fa8fc69b381/animals-11-01344-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/8151427/386c6b323baf/animals-11-01344-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/8151427/b4bf07e5bd24/animals-11-01344-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/8151427/700dc87b36ff/animals-11-01344-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/8151427/24624740bbcf/animals-11-01344-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/8151427/6fa8fc69b381/animals-11-01344-g005.jpg

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