建立牛胚胎干细胞的策略。

Strategy to Establish Embryo-Derived Pluripotent Stem Cells in Cattle.

机构信息

Department of Bioengineering and QB3, UC Berkeley, Berkeley, CA 94720, USA.

Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, Seoul National University School of Dentistry, Seoul 08826, Korea.

出版信息

Int J Mol Sci. 2021 May 9;22(9):5011. doi: 10.3390/ijms22095011.

DOI:10.3390/ijms22095011

PMID:34065074

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8125899/

Abstract

Stem cell research is essential not only for the research and treatment of human diseases, but also for the genetic preservation and improvement of animals. Since embryonic stem cells (ESCs) were established in mice, substantial efforts have been made to establish true ESCs in many species. Although various culture conditions were used to establish ESCs in cattle, the capturing of true bovine ESCs (bESCs) has not been achieved. In this review, the difficulty of establishing bESCs with various culture conditions is described, and the characteristics of proprietary induced pluripotent stem cells and extended pluripotent stem cells are introduced. We conclude with a suggestion of a strategy for establishing true bESCs.

摘要

干细胞研究不仅对于人类疾病的研究和治疗至关重要，而且对于动物的遗传保存和改良也至关重要。自从在小鼠中建立了胚胎干细胞（ESCs）以来，人们已经做出了大量努力来在许多物种中建立真正的 ESCs。尽管已经使用各种培养条件来在牛中建立 ESCs，但尚未捕获到真正的牛胚胎干细胞（bESCs）。在本综述中，描述了使用各种培养条件建立 bESCs 的困难，并介绍了专有的诱导多能干细胞和扩展多能干细胞的特征。最后，我们提出了建立真正的 bESCs 的策略建议。